摘要
研究细菌的生长规律,快速获取细菌在生长过程中浓度、结构、化学组分等特征参数的变化,能够为环境评估、微生物研究等领域的细菌快速检测提供依据。采用紫外-可见光分光光度法测定水体常见大肠埃希氏菌(大肠杆菌)在生长过程中240~900nm波段的透射光谱,基于Mie散射理论对测量得到的可见光波段的透射光谱进行解析,得到细菌的浓度和大小等信息;以核酸、发色团氨基酸吸收特性为基础,根据细菌紫外吸收光谱计算了细菌的核酸含量。结果表明:通过透射光谱反演出的参数能够反映细菌在生长过程中浓度、大小和化学组分的变化,这些变化表明大肠杆菌在适宜的生长条件下经历了不同的生长时期,符合细菌生长规律。多波长透射光谱技术可以对细菌在生长过程中进行动态跟踪检测,获得细胞形态学和化学组分信息,为研究细菌多参数测量提供了一种新方法。
Research on the laws of bacterial growth and rapid acquisition of the changes in concentration,structure and chemical composition of bacterial cells can provide evidence for fast bacterial detection in environmental assessment and microbiological research fields.The transmission spectra in the region of 240-900 nm of Escherichia coli suspension during growth are recorded by ultraviolet-visible spectrophotometry.The measured transmission spectra in the visible regions are interpreted based on Mie scattering theory,and the information of concentration and size of bacteria during growth are acquired.According to the ultraviolet absorption spectra of bacteria,the nucleic acid content is calculated with the absorption characteristics of chromophore amino acid and nucleic acid.The results show that the parameters interpreted from the spectra can reflect changes in concentration,size and chemical composition of bacteria during growth.These changes demonstrate that Escherichia coli cells undergo different growth stages under suitable growth condition,which is consistent with the laws of bacterial growth.Multi-wavelength transmission spectroscopy can realize bacteria motion-tracking detection during growth,and can obtain morphological characteristics and chemical composition of bacteria,which provides a new method for multiple parameters measurement of bacteria.
作者
胡玉霞
赵南京
甘婷婷
段静波
刘建国
刘文清
Hu Yuxia1,2,3, Zhao Nanjing1,3, Gan Tingting1,3, Duan Jingbo1,3, Liu Jianguo1,3, Liu Wenqing1,3(1Key Laboratory of Environment Optics and Technology, Anhui Institute of Optics and Fine Mechanics Chinese Academy of Sciences, Hefei, Anhui 230031, China ; ZUniversity of Science and Technology of China, Hefei, Anhui 230026, China ; 3Anhui Key Laboratory of Environment Optical Detection Technology, Hefei, Anhui 230031, Chin)
出处
《中国激光》
EI
CAS
CSCD
北大核心
2018年第2期268-274,共7页
Chinese Journal of Lasers
基金
安徽省自然科学基金(1508085JGD02
1608085QF137)
安徽省科技重大专项(15CZZ04125)
国家自然科学基金(61378041
61705237)
关键词
生物光学
光谱学
MIE散射
多波长透射光谱
细菌检测
细菌生长
biotechnology
spectroscopy
Mie scattering
multi-wavelength transmission spectroscopy
bacteriadetection
bacteria growth
