摘要
目的观察肺痹方对博来霉素诱导的肺纤维化小鼠抗氧化作用,并探讨其机制。方法将60只C57BL/6雄性小鼠随机分为空白对照组、模型组、吡非尼酮组[51.43 mg/(kg·d)]、肺痹方低剂量组[6.43 g/(kg·d)]、肺痹方中剂量组[12.86 g/(kg·d)]、肺痹方高剂量组[25.72g/(kg·d)],每组10只。除空白对照组外,其余各组按7.5 mg/(kg·d)腹腔注射博来霉素,空白对照组腹腔注射等量的生理盐水,连续注射10 d。造模后第2天灌胃给药,空白对照组和模型组给予等量生理盐水灌胃,给药时间为4周。每周称取各组小鼠的体质量,监测小鼠的一般情况。实验结束后,计算肺纤维化小鼠的肺系数,取右肺中叶测定各组小鼠肺组织均浆中的羟脯氨酸(HYP)、超氧化物歧化酶(SOD)、还原谷胱甘肽(GSH)、丙二醛(MDA)的含量。取小鼠左肺,用于HE染色和Masson染色,观察其组织病理改变。结果与其他各组相比,模型组肺泡炎和肺纤维化程度明显增加,肺系数和肺组织HYP、MDA的含量明显升高,肺组织SOD、GSH含量明显降低(P<0.05或P<0.01);与模型组相比,各给药组可明显改善肺纤维化小鼠肺泡炎及肺纤维化程度,降低肺系数、肺组织中HYP、MDA的含量(P<0.05或P<0.01),升高肺组织中SOD、GSH的活性。结论肺痹方具有良好的抗炎、抗氧化作用,对博来霉素诱导肺纤维化小鼠具有一定治疗作用。
Objective： To investigate the antioxidant effects of Feibi Decoction on mice with pulmonary fibrosis and to explore its mechanism. Methods： 60 male C57 BL/6 mice were randomly divided into the control group,the model group,pirfenidone group [51.43 mg/（kg·d）],Feibi Decoction low dose group [6.43 g/（kg·d）],Feibi Decoction middle dose group [12.86 g/（kg·d）],Feibi Decoction high dose group [25.72 g/（kg·d）],10 mice in each group. Except the control group,the other groups were injected with 7.5 mg/（kg·d） intraperitioneal injection of bleomycin,and the control group was injected with equal amount of normal saline for 10 days. On the second day after molding,the mice were treated with daily intragastric administration. The mice in control group and model group were treated with the same volume of normal saline,which lasted for 4 weeks. The weight of mice in each group was measured each week and the general condition of the mice was monitored. After the experiment,the lung coefficients of mice with pulmonary fibrosis were calculated;the content of HYP,SOD,reduced GSH and MDA in the lung homogenate of each group were determined. The left lung of mice was taken for HE staining and Masson staining to observe the histopathological changes. Results： Compared with other groups,the alveolitis and pulmonary fibrosis in the model group were significantly increased;the lung coefficient,the content of HYP and MDA in the lung tissue was significantly increased;the content of SOD and GSH in the lung tissue was significantly decreased（P〈0.05 or P〈0.01）. Compared with the model group,each administration group could significantly improve alveolitis and pulmonary fibrosis in mice with pulmonary fibrosis,reduce the lung coefficient,the levels of HYP and MDA（P〈0.05 or P〈0.01） and increase the activity of SOD and GSH in lung tissue. Conclusion： Feibi Decoction has a good anti-inflammatory and antioxidant effect and a certain therapeutic effect on bleomycin induced pulmonary fibrosis in mice.
作者
程雪
方泓
吴银根
CHENG Xue,FANG Hong(Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine ,Shanghai 200032, Chin)
出处
《中国中医急症》
2018年第3期394-397,409,共5页
Journal of Emergency in Traditional Chinese Medicine
基金
国家中医药管理局全国名老中医药专家传承工作室建设项目(LH02.07.005)
关键词
肺纤维化小鼠
肺痹方
抗氧化
Pulmonary fibrosis in mice
Feibi Decoction
Antioxidant
