摘要
目的:建立博莱霉素导致肺间质纤维化小鼠动物模型,比较不同给药方式的成模差异。方法:利用8周龄雄性ICR小鼠,①随机分为腹腔给药组(P组)、气管内给药组(I组)、阴性对照组(C组),分别经腹腔注射BLM 40 mg/kg 5次、气管内滴入BLM 5 mg/kg 1次或气管内滴入生理盐水50μl。分别于14、28、40天处死,②小鼠随机分为4组,分别经腹腔注射BLM 40mg/kg3、4、5次或经腹腔给予生理盐水200μl。分别于28、40天处死。观察小鼠体重、咳嗽、挠鼻症状、肺系数及肺组织病理改变。结果:给予博莱霉素后①小鼠的体重均下降并出现咳嗽及挠鼻等呼吸障碍症状;处置后第14、28及40天处死小鼠,计算肺系数,P组较I组肺系数高;处死小鼠后,P组和I组小鼠均形成广泛、稳定的间质纤维化病理改变,P组主要分布在胸膜下及血管周围,而I组主要分布在肺门和支气管周围。P组较I组肺纤维化病理评分高。②不同腹腔给药次数模型小鼠体重变化以5次给药对体重影响最大;计算肺系数以给药5次肺系数变化最大。上述模型均成功建立。通过比较生存率、呼吸困难症状、组织病理变化等指标,选出腹腔给药5次相对于给药3次及4次为更好的造模方式。结论:利用BLM腹腔注射和气管内滴入制备了肺间质纤维化动物模型,纤维化形成的部位存在着一定的差异,腹腔给药5次方法制备肺间质纤维化模型的成功率更佳。
Objective： To study the differences of bleomycin-induced pulmonary fibrosis in mice induced by intraperitoneal injection and intratracheal instillation of bleomycin. Methods： ICR mice （male, 8 w of age, 18 to 22 g bodyweight） were used. ①ICR mice were randomly divided into three groups. In the group P, bleomycin was injected intraperitoneal five times in a dose of 40 mg/kg, and in the group I, bleomycin was instilled intratracheally in a dose of 5 mg/kg. The mice were killed at 14,28 or 40 day. ②ICR mice were randomly divided into four groups ： bleomycin was injected intraperitoneal three, four or five times in a dose of 40 mg/kg, and in the group control , bleomycin was injected intraperitoneal in a dose of 200 μl for five times. The mice was killed at 28 or 40 day. The pathological changes and symptoms were observed. Results： ①The weight of the mice given blemycine were lost after injection. Symptoms were more serious in group P than those of group Ⅰ. The pulmonary coefficient of group P was more than that of group Ⅰ. At 28 day after injection, fibrosis was widely and stably formed mainly in around the bronchia and bronchioles, especially, near the pulmonary hilar area, in the group I mice, however, the same changes were mainly seen under the pleura or perivasenlar pulmonary tissues in the mice of group P. The pathological score of pulmonmy fibrosis was different between those two groups. ②Different dose of bleomycine induced different change of the mouse＇s weight. The most of all of the three group were five time injection; The lung index of five time injection of bleomycine was the most. The pulmonary fibrosis mouse model was made successfully. After comparising all of the data, we found intraperitoneal of bleomycine five times was the more convenient method. Conclusion： The sites of pulmonary fibrosis in the mice are different between the mice induced by intraperitoneal injection or intratracheal instillation of bleomycin. Intraperitoneal injection five times is a more convenient
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2010年第3期254-257,共4页
Chinese Journal of Immunology
