摘要
目的:研究电针胃俞募穴调节功能性消化不良(functional dyspepsia,FD)模型大鼠胃运动的中枢分子机制。方法:选用SD大鼠30只,随机分为空白组、模型组、中脘+胃俞组、胃俞组和中脘组5组,每组6只。除空白组外,其余大鼠均采用适度夹尾激怒法与不规则喂养法两种经典方法联合应用建立FD大鼠模型。中脘+胃俞组、胃俞组、中脘组相应选取"中脘"+"胃俞""胃俞""中脘"穴进行电刺激,每次20 min,每日1次,连续干预7 d。空白组与模型组不施加干预措施,模型组抓取固定。采用胃肠压力换能器记录大鼠胃窦部胃运动幅度及频率,Western blotting法测定大鼠迷走神经背核(dorsal motor nucleus of the vagus,DMV)区N-甲基-D-天冬氨酸受体(N-methyl-D-aspartate recepter,NMDAR)亚基NR1表达,酶联免疫吸附法(ELISA)法检测各组大鼠血清一氧化氮(nitric oxide,NO)含量。结果:与空白组比较,模型组胃窦运动幅度降低,DMV区NR1表达降低,血清NO含量增高(均P<0.05);与模型组比较,电针各组胃窦运动幅度增高,DMV区NR1表达增高,血清NO含量降低(均P<0.05);与中脘组、胃俞组比较,中脘+胃俞组胃窦运动幅度、DMV区NR1表达均增高(均P<0.05);与中脘+胃俞组、中脘组比较,胃俞组NO含量降低(均P<0.05)。模型组和空白组、电针各组胃窦运动频率组间比较差异无统计学意义(均P>0.05)。结论:电针胃俞募穴能够调节FD模型大鼠胃运动,可能是通过改变中枢DMV区NMDAR活性从而调节血清NO含量发挥作用的。
Objective To research the central molecular mechanism of gastric motility in functional dyspepsia（FD） rats treated with electroacupuncture（EA） at shu and mu points of stomach. Methods A total of 30 SD rats were randomized into a blank group, a model group, a Zhongwan＋Weishu group, a Weishu group and a Zhongwan group, 6 rats in each group. FD rats were established by moderate clipping tail infuriation and irregular feeding except in the blank group. EA was used at ＂Zhongwan＂（CV 12）,＂Weishu＂（BL 21）, and＂Zhongwan＂（CV 12） ＋＂Weishu＂（BL 21） in the corresponding groups for 7 days, once a day, and 20 min a time. No intervention was used in the blank and model groups. Grabbing and fixation were applied in the model group. Gastric antrum motion range and frequency were recorded by gastrointestinal pressure transducer. The expression of subunit NR1 of N-methyl-D-aspartate recepter（NMDAR） in dorsal motor nucleus of the vagus（DMV） was determined by Western blotting. The content of serum nitric oxide（NO） was measured by ELISA. Results Compared with the blank group, the gastric antrum motion range and NR1 in the DMV decreased and the serum NO content increased in the model group（all P〈0.05）. Compared with the model group, the gastric antrum motion range and NR1 in the DMV increased and the serum NO content decreased in the three EA groups（all P〈0.05）. Compared with the Zhongwan and Weishu groups, the gastric antrum motion range and NR1 in the DMV increased in the Zhongwan ＋ Weishu group（all P〈0.05）.Compared with Zhongwan ＋ Weishu and Zhongwan groups, the expression of NO in the Weishu group decreased（both P〈0.05）. The gastric antrum motion frequency among the 5 groups had no statistical significance（all P〈0.05）. Conclusion EA at the shu and mu points can regulate the gastric motility in FD rats which may be by modulating the activity of NMDAR in the central DMV region, thus regulating the serum NO content.
出处
《中国针灸》
CAS
CSCD
北大核心
2018年第3期285-290,共6页
Chinese Acupuncture & Moxibustion
基金
国家自然科学基金项目：81473784
安徽省自然科学基金项目：1408085 MH 166
安徽中医药大学校基金面上项目：2016 zr 002
安徽中医药大学研究生科技创新基金重点项目
安徽高校科研创新平台建设项目：2015 TD 033
关键词
功能性消化不良
电针
穴
胃俞
穴
中脘
N-甲基-D-天冬氨酸受体
胃运动
一氧化氮
functional dyspepsia
electroacupuncture
Point BL 21 （Weishu）
Point CV 12 （Zhongwan）
N-methyl-D-aspartate recepter （NMDAR）
gastric motility
NO
