摘要
本试验以6种盐溶液(NaCl,Na_2SO_4,KCl,K_2SO_4,CaCl_2,NaHCO_3)对紫花苜蓿种子进行胁迫处理,研究不同盐胁迫下紫花苜蓿种子的发芽率、发芽指数、平均发芽时间、盐害率及根长抑制率的差异。结果显示,紫花苜蓿种子的发芽率、发芽指数随盐溶液浓度增加而降低;平均发芽时间、盐害率和根长抑制率随盐浓度的升高而增加。通过分析得出紫花苜蓿种子发芽过程中对不同盐胁迫的响应浓度分别为:NaCl 25 mmol/L,K_2SO_450 mmol/L,CaCl_2100 mmol/L;耐盐极限浓度分别为:NaCl 190.97 mmol/L,Na_2SO_492.84 mmol/L,KCl353.86 mmol/L,K_2SO_4160.68 mmol/L,CaCl2260.55 mmol/L,NaHCO_3114.77 mmol/L。紫花苜蓿种子萌发过程中对不同盐分胁迫的耐受性大小依次为:NaHCO)3>Na_2SO_4>K_2SO_4>NaCl>KCl>CaCl_2。
In the present study,the effects of salinity on seed germination of Medicago sativa were evaluated by treated with six different types salt solution： NaCl,Na2SO4,KCl,K2SO4,CaCl2,NaHCO3.Germination rate,germination index,mean germination time,salt injury rate and relative inhibitory ratio of root length under different salts and different salt concentration have been investigated.The result showed that with increasing salt concentrations the germination rate and germination index of M.sativa seeds significantly decreased compared with the control.Meanwhile,the mean germination time,salt injury rate and relative inhibitory ratio of root length significantly increased compared with the control.The initial response salt concentration of M.sativa was： NaCl 25 mmol/L,K2SO450 mmol/L,CaCl2100 mmol/L.The limiting salt solution concentration was： NaCl190.97 mmol/L,Na2SO492.84 mmol/L,KCl 353.86 mmol/L,K2SO4160.68 mmol/L,CaCl2260.55 mmol/L,NaHCO3114.77 mmol/L.The tolerance of M.sativa seeds was different for the different salt type.The order of tolerance was： NaHCO3〉Na2SO4〉K2SO4〉NaCl 〉KCl〉CaCl2.
出处
《分子植物育种》
CAS
CSCD
北大核心
2018年第1期268-274,共7页
Molecular Plant Breeding
基金
青海省科技计划项目(2014-NK-A4-2-1)
国家自然科学基金项目(31300269)共同资助
关键词
紫花苜蓿
盐胁迫
种子萌发
发芽率
发芽指数
Medicago sativa
Salt stress
Seed germination
Germination rate
Germination index
