摘要
目的：利用均匀设计法分析铁元素对虫草素产量的影响。方法：采用均匀设计实验,以虫草素含量为检测标准,考察Fe2＋添加量、Fe3＋添加量及添加时间对虫草素产量的影响,并确定最佳方案。结果：利用均匀设计法得到的最佳实验方案为当Fe2＋浓度为1.5 g·L^（-1）,Fe3＋浓度为1.3 g·L^（-1）,第10天添加时可在每升蛹虫草发酵培养液中得到910.7 mg虫草素,与空白对照组相比含量提高了36.32%。结论：在培养基中添加铁元素会提高液体发酵蛹虫草产虫草素的产量。
Objective： To analyze the influence of iron elements on Cordycepin content by the uniform design. Methods： By using uniform design experiment we investigated the effects of Fe2 ＋addition,add time and Fe3 ＋addition on cordycepin content.Then the optimal experimental program was determined. Results： We could gain 910. 7 mg cordycepin in per liter of cordyceps militaris fermentation medium when the concentration of Fe2 ＋was 1. 5 g·L^（-1）,Fe3＋1. 3 g·L^（-1）. On 10 th day,compared with the blank control group,the cordycepin content increased by 36. 32%. Conclusion： The cordycepin content in the liquid fermentation can be improved by addition of iron elements.
出处
《辽宁中医杂志》
CAS
2018年第1期135-137,共3页
Liaoning Journal of Traditional Chinese Medicine
基金
山东省重点研发计划(520864)
关键词
均匀设计法
铁元素
虫草素
Keywords ： uniform design
iron element
cordycepin
