摘要
目的研究顺铂(DDP)联合食管癌相关基因2(ECRG2)蛋白对人食管癌EC9706细胞增殖抑制作用及诱导其凋亡的机制。方法将人食管癌EC9706细胞分为空白对照组、阳性对照组和低、中、高3个质量浓度实验组。阳性对照组细胞用3 mg·L^(-1)顺铂处理,低、中、高3个质量浓度实验组细胞在阳性对照组的基础上用质量浓度为5.5,7.5,9.5μg·L^(-1)的ECRG2蛋白处理,空白对照组细胞不做任何处理。检测各组细胞的活性和细胞凋亡率,用反转录-聚合酶链式反应(RT-PCR)法检测人食管癌EC9706细胞耐药基因的表达情况。结果空白对照组、阳性对照组和低、中、高3个质量浓度实验组人食管癌EC9706细胞活性分别为100.00%,(92.12±2.36)%,(88.62±3.22)%,(82.15±2.05)%,(78.41±2.16)%,阳性对照组和3个质量浓度实验组细胞活性显著低于空白对照组(P<0.05或P<0.01),中、高质量浓度实验组均显著低于阳性对照组(均P<0.05);且中、高质量浓度实验组细胞活性随ECRG2蛋白作用浓度增大逐渐降低(P<0.01)。阳性对照组和低、中、高3个质量浓度实验组人食管癌EC9706细胞凋亡率分别为(5.21±0.24)%,(11.45±0.66)%,(16.86±1.42)%,(22.62±2.12)%,均显著高于空白对照组的(2.89±0.32)%,低、中、高3个质量浓度实验组细胞凋亡率均明显高于阳性对照组(均P<0.01)。阳性对照组和低、中、高3个质量浓度实验组细胞谷胱甘肽巯基转移酶-π(GST-π)和多药耐药蛋白基因(MRP)mRNA相对表达量均低于空白对照组,且低、中、高3个质量浓度实验组低于阳性对照组(P<0.05或P<0.01)。结论顺铂可增强ECRG2蛋白对人食管癌EC9706细胞的增殖抑制作用,并可促进细胞凋亡,其机制可能与顺铂联合ECRG2显著降低GST-π和MRP基因表达有关。
Objective To investigate the inhibitory effect of cisplatin（ DDP） combined with esophageal cancer associated gene 2（ ECRG2）on the proliferation of human esophageal carcinoma EC9706 cells and its mechanism. Methods Human esophageal carcinoma EC9706 cells were divided into blank control group,positive control group and low,medium and high concentration experimental groups. Positive control group cells were treated with 3 mg · L^-1 cisplatin. The cells in low,medium and high concentration groups were treated with 5. 5,7. 5 and 9. 5 μg·L^-1 ECRG2,blank control group cells was given no treatment. The activity and apoptosis rate of cells in each group were detected, and theexpression of multi-drug resistance gene in human esophageal carcinoma cell line EC9706 was detected by reverse transcriptase-polymerase chain reaction（ RT-PCR）. Results The cell viability of EC9706 cells in control group,positive control group and low,medium and high concentration experimental groups were 100. 00%,（ 92. 12 ± 2. 36） %,（ 88. 62 ± 3. 22） % and（ 82. 15 ± 2. 05） %,（ 78. 41 ± 2. 16） %. The cell viability in positive control group and three experimental groups were significantly lower than that in blank control group（ P〈0. 05 or P〈0. 01）,and those in medium and high concentration groups were significantly lower than that in positive control group（ P〈0. 05）. The cell viability in medium and high concentration groups decreased with the increasing of ECRG2 protein concentration（ P〈0. 01）. The apoptotic rates of EC9706 cells in positive control group,low,medium and high concentration experimental groups were（ 5. 21 ± 0. 24） %,（ 11. 45 ± 0. 66） %,（ 16. 86 ± 1. 42） % and（ 22. 62 ± 2. 12） %,had significant difference with that in blank control group,which was（ 2. 89 ± 0. 32） %. The apoptotic rates of low,medium and high concentration experimental groups were significantly higher than that in pos-itive control group（ P〈0. 01）. The relative expression levels of GS
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2018年第4期446-449,共4页
The Chinese Journal of Clinical Pharmacology
关键词
食管癌
顺铂
食管癌相关基因2
增殖
凋亡
esophageal cancer
cisplatin
esophageal cancer related gene 2
proliferation
apoptosis
