摘要
目的：观察参芪扶正注射液（参芪液）对人肺腺癌顺铂耐药细胞株（A549/DDP）耐药性的逆转作用及其可能机制。方法：1~120 g/L参芪液干预A549/DDP细胞48 h,倒置显微镜观察细胞的形态变化;MTT法检测参芪液对A549/DDP细胞株增殖的作用以及对顺铂（DDP）敏感性的影响;低浓度参芪液（8 g/L）、中浓度参芪液（16 g/L）、高浓度参芪液（32 g/L）联合DDP（40μg/ml）分别作用于A549/DDP细胞24 h,流式PI单染法检测细胞凋亡率,Western blot检测Cleaved-caspase-3蛋白表达。结果：与对照组比较,2~120 g/L参芪液对A549/DDP细胞有增殖抑制作用,且呈浓度依赖性（P〈0.05）;2 g/L参芪扶正注射液作用A549/DDP细胞后,顺铂敏感性提高,逆转倍数（RF）为2.66倍;与DDP组比较,低、中、高浓度参芪液联合DDP组,均能显著诱导A549/DDP细胞凋亡（P〈0.05）,Cleaved-caspase-3蛋白表达上调。结论：参芪扶正注射液能够逆转A549/DDP细胞对顺铂的耐药性,其机制可能是通过启动凋亡调控基因介导的Caspase级联反应,活化Caspase-3,从而导致细胞凋亡。
Objective： To investigate the reverse effect and mechanisms of Shen Qi Fu Zheng injection（ SQFZI） on the cisplatin resistance of human lung adenocarcinoma cancer cell line（ A549 / DDP）. Methods： 48 h after treatment A549 / DDP cell with 1-120 g / L SQFZI,inverted microscope was used to observe the morphological changes of A549 / DDP cell line; MTT assay was used to determine the effect of SQFZI on proliferation and sensitiveness to cisplatin of A549 / DDP cell. 24 h after treatment A549 / DDP with low-（ 8 g / L）,middle-（ 16 g / L）,high-（ 32 g / L） concentration of SQFZI combined with DDP（ 40μg /m L）,PI Flow cytometry test and Western blot were used to determine cell apoptosis and Cleaved-caspase-3 expression respectively. Results： Compared with control group,2 ~ 120 g / L SQFZI can inhibit the proliferation of A549 / DDP cell in a concentration-dependent manner（ P 〈 0. 05）. Treated with 2 mg / m L SQFZI,the sensitivity of A549 / DDP cell line to cisplatin increased and the reversal fold up to2. 66. Compared with DDP group,SQFZI at different concentration combined with DDP significantly induced apoptosis in A549 / DDP cells（ P 〈 0. 05） and the expression of Cleaved-caspase-3 was up-regulated.Conclusion： SQFZI could moderately reverse the drug resistance of A549 / DDP cells to cisplatin. The mechanism may be related to start-up Caspase cascade reaction mediated by apoptosis regulation gene and activate Caspase-3,which leads to apoptosis.
出处
《上海中医药大学学报》
CAS
2016年第4期52-56,共5页
Academic Journal of Shanghai University of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(81573856
81473569)
辽宁省自然科学基金项目(2015020379)
