摘要
目的建立分散微固相萃取-同位素内标法-超高效液相色谱-串联质谱测定水果中吗啉残留量的检测方法。方法样品经含1%甲酸的乙腈提取,经分散微固相萃取净化、超高效液相色谱BEH HILIC色谱柱分离后,经串联质谱检测,同位素内标法定量。结果吗啉在0~200μg/L浓度范围内线性关系良好,线性相关系数为0.9998。相对标准偏差(relative standard derivation,RSD)为1.2%~2.9%,加标回收率为93.0%~101.6%。结论本方法采用同位素内标法,具有灵敏度高、精密度和准确度好等优点,简便快捷,适合水果中吗啉的日常检测工作。
Objective To establish a method for determination of morpholine in fruits by dispersive micro solid phase extraction-isotope internal standard-ultra performance liquid chromatography tandem mass spectrometry （UPLC-MS-MS）. Methods Samples were extracted by acetonitrile containing 1% formic acid, and purified by dispersive micro solid phase extraction （DMSPE）. The analyte was separated on a BEH HILIC column, detected by mass spectrometry and quantified by isotope internal standard method. Results It showed good linear relationship in range of 0~200μg/L for morpholine （r=0.9998）. The relative standard derivations were 1.2%~2.9%, and the recoveries were 93.0%-101.6%. Conclusion This method use isotope internal standard method, and it is rapid, sensitive, and selective with excellent precision and accuracy, which can be used for daily detection of morpholine in fruits.
出处
《食品安全质量检测学报》
CAS
2018年第4期918-924,共7页
Journal of Food Safety and Quality
关键词
超高效液相色谱-串联质谱法
同位素内标法
分散微固相萃取
吗啉
水果
ultra performance liquid chromatography tandem mass spectrometry
isotope intemal standard method
dispersive micro solid phase extraction
morpholine
fruits
