摘要
胶原超细纤维膜在外用敷料、人工皮肤等医用领域具有潜在的应用前景,为解决其结构不稳定、遇水易溶解等问题,采用柠檬酸对胶原进行原位交联,再进行静电纺丝制得超细纤维膜,并对纤维膜形貌、水溶解性、化学结构、交联性和细胞毒性等进行测试与评价。结果表明,质量分数为10%的柠檬酸原位交联的牛肌腱胶原超细纤维膜,其交联度可达30%,水溶解性能明显降低,化学结构无明显变化,无生物毒性,可用于生物医用领域。
The microfiber membrane of collagen has potential applications in medical fields, such as wound dressing, artificial skin. In order to solve problems of the collagen structures lacking of stability and easy dissolving in water, the citric acid was used to make in-situ crosslink with collagen, and then the superfine fiber membrane was obtained through electrospinning. The morphology, water solubility, chemical structure, degree of cross-linking and cytotoxicity of fiber membranes were tested and analysed. The results show that the degree of crosslinking of bovine tendon collagen membrane in-situ crosslinked with 10% citric acid is up to 30%. The water solubility of collagen membrane is obviously reduced, while the chemical structure of the collagen membrane before and after crosslinking has no evident change. All the crosslinked samples are non-biotoxicity, indicating that the crosslinked collagen membranes can be used in biomedical field.
出处
《东华大学学报（自然科学版）》
CAS
CSCD
北大核心
2018年第1期38-44,73,共8页
Journal of Donghua University(Natural Science)
基金
上海市科技成果转化资助项目(14441901600)
江苏省科技支撑资助项目(BE2014036)
关键词
胶原
静电纺
柠檬酸原位交联
性能表征
collagen
electrospinning
citric acid in-situ crosslinking
characterization
