摘要
目的·观察补骨脂素和异补骨脂素对人牙周膜细胞炎症因子表达的影响。方法·组织块法原代培养人牙周膜细胞。CCK-8试验检测2种药物对人牙周膜细胞活性的影响。用不同浓度的补骨脂素和异补骨脂素预处理人牙周膜细胞2 h后,再用牙龈卟啉单胞菌脂多糖(Porphyromonas gingivalis lipopolysaccharide,Pg-LPS)刺激,real-time PCR和ELISA分别检测白介素-1β(interleukin-1β,IL-1β)和白介素-8(IL-8)的mRNA和蛋白表达。结果·采用组织块法可成功培养出人牙周膜细胞。12.5μg/mL及以下浓度的补骨脂素和异补骨脂素对人牙周膜细胞活性无影响。补骨脂素和异补骨脂素不仅可下调Pg-LPS诱导的人牙周膜细胞IL-1β和IL-8 mRNA的表达,还可抑制IL-1β和IL-8蛋白的分泌。结论·补骨脂素和异补骨脂素可显著抑制Pg-LPS诱导的人牙周膜细胞炎症因子的表达,可用于牙周炎的治疗和预防。
Objective·To investigate the effects of psoralen and angelicin on inflammation cytokine expression of human periodontal ligament cells(hPDLCs). Methods·hPDLCs were primarily cultured using tissue explant method. Effects of psoralen and angelicin on the cell viability were tested by CCK-8 assay. h PDLCs were stimulated by Porphyromonas gingivalis lipopolysaccharide(Pg-LPS) after treatment with different concentrations of psoralen and angelicin for 2 h. m RNA expression of IL-1β and IL-8 were determined by real-time PCR. Enzyme-linked immunosorbent assay(ELISA) was used to measure the secretion of IL-1β and IL-8. Results·hPDLCs were cultured successfully by tissue explant method. Psoralen and angelicin( ≤12.5 μg/mL) did not show significant effects on the cell viability of h PDLCs. Pg-LPS markedly elevated the mRNA expression of IL-1β and IL-8, which could be attenuated by psoralen and angelicin in a dose-dependent manner. Likewise, the up-regulated protein secretion of IL-1β and IL-8 could be significantly blocked by psoralen and angelicin. Conclusion·Psoralen and angelicin could attenuate the inflammatory response of hPDLCs induced by Pg-LPS. Therefore, psoralen and angelicin may act as natural agents to prevent and treat periodontitis.
出处
《上海交通大学学报（医学版）》
CAS
CSCD
北大核心
2018年第2期128-132,共5页
Journal of Shanghai Jiao tong University:Medical Science
基金
国家自然科学基金(81670992
81271156)
上海交通大学医工交叉项目(YG2015MS04)~~
关键词
人牙周膜细胞
补骨脂素
异补骨脂素
抗炎
human periodontal ligament cells
psoralen
angelicin
anti-inflammation
