摘要
目的:观察隔药饼灸对兔动脉粥样硬化(AS)斑块中Toll样受体2(TLR 2)、TLR 4、核转录因子κB(NF-κB)mRNA表达的影响,探讨隔药饼灸稳定兔AS斑块的作用机制。方法:新西兰大耳白兔随机分为空白组、模型组、直接灸组、隔药饼灸组、药物组,每组12只。采用高脂饲料喂养及腹主动脉球囊损伤法建立兔AS模型。直接灸组及隔药饼灸组分别直接灸及隔药饼灸"巨阙"及双侧"天枢""丰隆"或双侧"心俞""脾俞""肝俞",每日1次,治疗8周。运用酶法测定血浆总胆固醇(TC)、甘油三酯(TG);比色法测定血浆高密度脂蛋白(HDL)、低密度脂蛋白(LDL),并计算HDL/LDL;电泳法测定血浆载脂蛋白A(Apo-A)、载脂蛋白B(Apo-B);HE染色观察腹主动脉斑块组织结构及斑块的血管内膜厚度、内膜中膜厚度(IMT);实时荧光定量PCR技术检测兔AS斑块中TLR 2、TLR 4、NF-κB mRNA的表达水平。结果:与空白组比较,模型组血浆TC、TG、LDL、Apo-B含量显著升高(P<0.01),Apo-A含量、HDL/LDL显著降低(P<0.01),主动脉内膜厚度、IMT显著增加(P<0.01),TLR 2、TLR 4、NF-κB mRNA表达水平显著升高(P<0.01)。与模型组比较,各治疗组血浆TC、TG、LDL、Apo-B含量显著降低(P<0.05,P<0.01),Apo-A含量、HDL/LDL显著升高(P<0.01),主动脉内膜厚度、IMT显著降低(P<0.01),TLR 2、TLR 4、NF-κB mRNA表达水平显著降低(P<0.05,P<0.01)。与直接灸组比较,隔药饼灸组和药物组血浆TC、TG、LDL、Apo-B显著降低(P<0.05,P<0.01),Apo-A含量、HDL/LDL显著升高(P<0.01),主动脉内膜厚度、IMT降低(P<0.01,P<0.05),TLR 2、TLR 4、NF-κB mRNA表达水平显著降低(P<0.05,P<0.01)。结论:隔药饼灸通过抑制兔AS斑块中TLR 2、TLR 4、NF-κB mRNA的表达,可能起到稳定AS斑块的作用。
Objective To observe the effect of herbal cake-separated moxibustion on blood lipid-apoprotein levels and the expression of Toll-like receptor 2（TLR 2）,TLR 4 and nuclear factor kappa B（NF-κB）mRNAs in atherosclerotic（AS）vulnerable plaques of hyperglycemia rabbits,so as to explore its mechanism underlying improvement of atherosclerosis.Methods Sixty New Zealand rabbits were randomly divided into 5 groups：control,model,direct moxibustion,herbal-cake-separated moxibustion and medication groups（n=12 rabbits in each group）.The AS vulnerable plaque model was established by high-fat forage feeding plus balloon-induced abdominal aorta injury and gene transfection of Ad 5-p 53 recombinant vector.Direct moxibustion or herbal-cake-separated moxibustion was applied to＂Juque＂（CV 14）and bilateral＂Tianshu＂（ST 25）,＂Fenglong＂（ST 40）,or bilateral＂Xinshu＂（BL 15）,＂Pishu＂（BL 20）and＂Ganshu＂（BL 18）for 15-20 min every time.The medication group was treated by feeding Atorvastatin.All the treatments were conducted once daily for 8 weeks.Plasma total cholesterol（TC）and triglyceride（TG）contents were detected by enzyme method,high-density lipoprotein（HDL）and low-density lipoprotein（LDL）contents detected by colorimetric determination,and plasma apolipoprotein A（Apo-A）and apolipoprotein B（Apo-B）levels determined by electrophoretic method.The pathological changes of vulnerable plaque and the aortic intima and media thickness were observed under light microscope after H.E.staining.The expression levels of TLR 2,TLR 4 and NF-κB mRNAs in AS plaques were determined by quantitative real-time PCR.Results After modeling,the levels of plasma TC,TG,LDL and Apo-B in the model group were remarkably increased（P0.01）,and Apo-A and HDL/LDL were significantly decreased in comparison with the control group（P0.01）.Additionally,the aortic intima and media thickness and the expression levels of TLR 2,TLR 4 and NF-κB mRNAs in AS plaques were significantly increased�
作者
郑雪娜
杨俊
谢涛
郭斌
谢志强
谢莉娜
吴雪芬
胡岗珠
刘迈兰
岳增辉
ZHENG Xue-na;YANG Jun;XIE Tao;GUO Bin;XIE Zhi-qiang;XIE Li-na;WU Xue-fen;HU Gang-zhu;LIU Mai-lan;YUE Zeng-hui(College of Acu-moxibustion and Massage,Hunan University of Chinese Medicin)
出处
《针刺研究》
CAS
CSCD
北大核心
2018年第2期92-97,共6页
Acupuncture Research
基金
国家重点基础研究发展计划(No.2014CB 543102)
国家自然科学基金项目(No.81273860
81704182)
关键词
隔药饼灸
动脉粥样硬化斑块
血浆-载脂蛋白
TOLL样受体2
Toll样受体4
核因子ΚB
Herbal-cake-separated moxibustion
Atherosclerotic vulnerable plaques
Plasma lipid-apolipoproteins
Toll-like receptor 2 （TLR 2）
Toll-like receptor 4 （TLR 4）
Nuclear factor kappa B （NF-κB）
