摘要
目前已有的动物模型在研究动脉粥样硬化斑块破裂、破裂的可控性及量化研究方面均不能满足研究的需要.为了建立类似于人类动脉粥样硬化病变的斑块模型,体外研究斑块稳定性,应用传统的高脂高胆固醇膳食诱导建立了小型猪动脉粥样硬化模型,并从血脂水平和斑块病理形态学特征方面加以了证实.该模型中斑块与人类成熟斑块的高度相似性使其成为研究斑块稳定性和斑块破裂的较好模型.从量化比较这一出发点着手,建立了一个体外可控可量化诱导斑块破裂模型,方法简单易行,是一个较好的量化研究斑块破裂和破裂相关因素间关系的实验模型.
The atherosclerotic animal model was established, whose pathological changes and the plasma lipid level are as same as the atherosclerotic injury in human, and then the stability of atherosclerotic plaque was studied. The miniswine resemble human in the physiology, biochemistry and anatomy. So it is a very appropriate candidate animal for atherosclerosis study. The miniswine atherosclerotic model by high lipid and high cholesterol diet was successfully established, whose plaque included a necrotic lipid core, a thin fibrin cap, a lot of foam cells and inflammatory cells. Calcify and intraplaque hemorrhage might be found in plaque. Based on the pathological character, the model might be as an appropriate tool for the study of plaque rupture and stability. Atherosclerotic plaque rupture was the major cause of the clinic cardiovascular diseases. To study the mechanics of plaque rupture had important significance. The establishment of the appropriate animal model of plaque rupture was the premiss to study the mechanism of plaque rupture. The established animal model existed deficiency on many aspects, such as uncontrolling and quantifying the pressure which led to plaque rupture, and whether the rupture was really existed. A swine model of plaque rupture was established in the experiment, which had much excellences, such as the pressure which led to plaque rupture could be controlled and qualified in vitro, and the way was very simple. So it provided a very good animal model for studying plaque rupture and the relation of rupture and related factors with rupture.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2006年第2期196-200,共5页
Progress In Biochemistry and Biophysics
基金
国际科技合作重点项目(2004DFA06400)
湖南省科技厅重点资助项目(01SSY1003).~~
关键词
动脉粥样硬化
斑块破裂
猪
动物模型
atherosclerosis, plaque rupture, minipig, animal model
