摘要
目的:基于小型猪的生理特点及循环系统与人类极为相似的特征,通过药物对其造模,建立一种新型的脉诊动物模型应用于脉诊教学中。方法:选用平均5个月龄,体重20±1.6kg,选取心率<100次/min小型猪6只,采用腹腔给予戊巴比妥钠进行麻醉,定位取脉位置,切脉后,用NX-8型脉诊仪同步采取正常脉图、二导心电图,耳大静脉心得安注射液及葡聚糖T-500静脉给药进行造模,观察造模后一般情况(心率、皮肤颜色)及指下脉型变化,同步描记脉图及二导心电图,涩变后记录数值:造模后3min、5min、10min、15min、20min、25min心率变化;主波高(HB)、升支斜率(Sup)、降支斜率(Sdown)、降中峡位置(即降中峡高/主波高HE/HB)、升支时间/脉波周期(TAB/TAG)、主波峰时间/脉波周期(TB/TAG)。结果:6头小型猪均造模成功,指下感觉涩变明显;脉图变化显著;与造模前相比,HB、Sup、Sdown、HE/HB、TAB/TAG、TB/TAG均有显著性差异(除SdownP<0.05外,其余均P<0.01)。讨论:通过运用小型猪成功建立脉涩模型发现,小型猪应用于脉诊教学实验方法简便、损伤小、可重复运用,有充分的可行性,以此为基础可以建立多种脉诊模型,以补充脉诊实验教学中的空白。
A mini- pig has a physiolog： text, it is worth to establish an an picked up six Chinese mini -pigs for heart rate）. Pentobarbital sod eal and circulatory system bearing much resemblance to that of humans. In this conimal based pulse model for the purpose of drug testing and teaching. In this study, we bred for experimental purpose （5 -6 months old, 20 ± 1.6kg, and 100 beats/minute ium was used for anesthesia, before pulse taking. Using an NX -8 multifunctional sphygmograph, we took the pressure sphygmogram and ECG 11. We also injected Propranolol and Dextran T- 500 in Meridian big ears, to form up a pulse diagnosis model. Minipig＇ s general reactions （heart rate and skin color） and finger＇s pulse variations were observed. Pressure sphygmogram and ECG 11 were taken simultaneously using the NX -8 multifunctional sphygmograph. The following parameters were also recorded ： Height of B wave （ HB）, Mean Slope up （Sup）, Mean Slope down （Sdown） ,Time between point A and E wave（HE/HB） ,Time of slope up/Pulse rate, and Time of B wave/Pulse rate（TB/TAG）. The value of modeling heart rate was measured at of 3rd, 5th, 10th, 15th, 20th, and 25th minute after ingection. The pulse model was successfully established in six mini -pigs. It can easily see that normal pulse be changed into unsmooth pulse in fingers. Sphygmogram also changed in HB,Sup,Sdown,HE/HB,TAB/TAG,TB/ TAG , compared with the values before modeling （ P 〈 0.01, except SDown at P 〈 0.05）. We believe that an animal based pulse model is desirable for the teaching purpose, thanks to its convenient, feasible, less - damaged, and repeatable features. That means establishing different animal pulse models will allow an enhanced effectiveness of pulse teaching.
出处
《世界科学技术-中医药现代化》
2007年第5期161-164,共4页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家自然科学基金面上项目(30371720):脉诊属性的检测技术与方法学探讨
国家自然科学基金重点项目(60431020):中医关键诊断信息获取与处理理论与技术研究
国家"十五"科技攻关计划项目(2004BA721A09):中医脉象信息采集关键技术研究
国家"十一五"科技支撑计划项目(2007BAI07A23):便携式辅助诊疗设备开发和导航针刀应用技术开发
关键词
小型猪
教学模型
脉涩
mini - pigs
teaching model
unsmooth pulse
