摘要
目的:观察超早期推拿对骨骼肌急性钝挫伤模型大鼠肌细胞膜修复相关蛋白dysferlin表达的影响,探寻dysferlin在骨骼肌钝挫伤肌细胞膜修复中的作用机制。方法:SD大鼠55只,随机分为正常对照组(n=5)、自然恢复组(n=25)、推拿组(n=25)。根据推拿治疗的天数计时,自然恢复组和推拿组分别在1d、2d、3d、5d、7d五个时间点处死,每亚组每个时间点5只,在腓肠肌标记的区域内取肌肉组织,HE观察组织的形态变化,运用Western blot检测蛋白dysferlin的表达情况,并进行统计学分析。结果:HE结果显示,与正常对照组相比,骨骼肌钝挫伤后,1d和2d肌纤维出现肿胀;3d出现炎性细胞浸润且自然恢复组比推拿组更加严重;5d炎性细胞浸润更加明显;7d推拿组炎性细胞较5d明显减少,结缔组织形成较少,7d自然恢复组有大量的结缔组织填充在肌纤维之间。WB结果显示:dysferlin在模型组中比在正常对照组中的表达明显增多;各个相应时间点推拿组较自然恢复组表达量明显增多(P<0.05),且随着时间的推移dysferlin在推拿组各亚组、自然恢复组各亚组中的表达量呈逐渐上升趋势。结论:在骨骼肌急性钝挫伤模型大鼠腓肠肌修复过程中,超早期推拿可增加膜修复蛋白dysferlin的表达,促进破裂的肌细胞膜及时修复,可能有利于损伤肌细胞的修复,从而帮助受损的骨骼肌修复。
Objective： To observe the effect of ultra-early massage on the expression of membrane repair associated protein dysferlin in rats with acute skeletal muscle injury , and demonstrate a possible mechanism how ultra-early massage protects myocytes from skeletal muscle contusion. Method： Fifty-five SD rats were randomly divided into normal control group （n=5）, natural recovery group （n=25） and massage group （n=25）. Rats in the natural recovery groups and the massage groups were sacrificed after treatment for 1d, 2d, 3d, 5d, 7d. There were 5 rats in subgroups at each time point. Muscle tissue was harvested in the region of the marked muscle. Morphological changes in the skeletal muscle tissues were observed using HE stain. The expression level of dysferlin was detected by Western blot. Result： HE： Compared with the normal control group, muscle fiber blunt swelling appeared at 1d and 2d after skeletal muscle contusion. Inflammatory cells of the natural recovery group was more than that of the massage group at 3d. More inflammatory cells were observed at 5d. Inflammatory cells in 7d massage group significantly decreased compared with that in 5d massage group. Less extensive fibrosis was observed in the massage group than the natural recovery group at 7d. WB： The expression level of dysferlin in the model groups was significantly higher than that in the normal control group. The expression of dysferlin in the massage group was significantly higher than that in the natural recovery group at each corresponding time （P〈0.05）. The level of dysferlin in the subgroups of the massage group and the natural recovery group presented gradual increase with the time goes on. Conclusion： In the course of acute contusion, ultra-early massage can increase the expression of dysferlin, promote the timely repair of ruptured membrane and decrease the muscle injury, which facilitate the repair of damaged skeletal muscle.
出处
《中国康复医学杂志》
CAS
CSCD
北大核心
2018年第2期151-157,共7页
Chinese Journal of Rehabilitation Medicine
基金
国家自然科学基金资助项目(81273870)
重庆市体育局科研项目(B201409)
关键词
推拿
骨骼肌钝挫伤
膜修复
CA^2+
massage
acute skeletal muscle injury
membrane repair
dysferlin
Ca^2＋
