摘要
口蹄疫病毒(foot-and-mouth disease virus,FMDV)主要通过呼吸道传播,切断呼吸道传播途径能有效的防御口蹄疫。本研究应用O型口蹄疫灭活病毒配合枯草芽胞杆菌通过喷鼻气雾免疫牛来探讨对牛呼吸道黏膜免疫应答及系统免疫应答的影响。结果显示,免疫后牛鼻黏膜与肺内支气管黏膜中的IgA分泌细胞的数量极显著增多(P<0.01),而常规口蹄疫灭活苗对IgA分泌细胞数量的影响不大(P>0.05);同时,ELISA结果显示鼻、气管、肺和肺内支气管中IL-12、TNF-α水平均极显著增加(P<0.01),尤其是在气管和肺内支气管中最为明显,但IL-6无明显差异(P>0.05)。从免疫第3天开始牛鼻液、唾液中口蹄疫特异性抗体SIgA以及血清中O型口蹄疫病毒特异性抗体均显著增加(P<0.01或P<0.05),且维持至3个月。本研究结果表明O型口蹄疫灭活病毒配合枯草芽胞杆菌喷鼻后可刺激牛呼吸道局部体液免疫和细胞免疫,同时还可诱导全身系统免疫应答,本研究为口蹄疫灭活病毒鼻腔免疫提供理论基础和应用前景。
Foot-and-mouth disease virus（FMDV）is the causative agent of contagious and economically devastating diseases that affects cloven-hoofed livestock worldwide.An effective defense barrier against FMD would be established if the respiratory tract transmission for virus was being cut off due to FMDV is mainly transmitted by the respiratory tract.In the current study,health cattle were immunized by inactivated FMDV along with Bacillus subtilis administered through intranasal spray to explore the impact on mucosal immune response（respiratory tract）and systemicimmunity in cattle.The results showed that the IgA secreting cells significantly increased in cattle nasal mucosae and pulmonary bronchial（P〈0.01）after intranasal immunization with inactivated FMDV along with Bacillus subtilis,but the conventional inactivated FMDV vaccine had no significant effect on the number of IgA secreting cells（P〉0.05）.At the same time,the level of IL-12 and TNF-αsignificantly increased in nose,trachea,pulmonary and pulmonary bronchus（P〈0.01）,especially in the trachea and pulmonary bronchus,but IL-6 level have no significantly difference（P〉0.05）.O type FMDV specific SIgA from nasal,saliva and O type FMDV specific antibodies in serum significantly increased at 3 days after intranasal immunization,and maintained for 3 months（P〈0.01 or P〈0.05）.These results indicated that both cellular immunity and local humoral immunity of the bovine respiratory tract were enhanced by intranasal immunization with inactivated FMDV along with Bacillus subtilis,as well as the systemic immune response.Our study provided an easier method and prospect for application of whole inactivated virus.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2018年第2期412-421,共10页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
关键词
口蹄疫灭活病毒
枯草芽胞杆菌
鼻腔免疫
黏膜免疫应答
inactivated foot-and-mouth disease virus
Bacillus subtilis
intranasal immunizations mucosal immune response
