摘要
为探究薄壳山核桃叶中的化感物质对植物生长的影响,采用室内种子发芽的方法,通过生物测定研究了石油醚相、氯仿相、乙酸乙酯相、甲醇相浸提液对高丹草种子萌发和幼苗生长的影响。结果表明,不同溶剂提取液对高丹草种子萌发和生长的影响不同。甲醇相对种子发芽率、发芽势、发芽指数、茎长起极显著的抑制作用,抑制指数分别达到0.13、0.13、0.23、0.61;对幼苗鲜重和根长起显著性抑制作用,抑制指数分别达到0.13、0.31。乙酸乙酯相对种子发芽指数也起到极显著抑制作用,抑制指数为0.13。而石油醚相、氯仿相对种子发芽势、平均发芽速度、根长、茎长等指标都有不同程度的影响,但差异都不显著。各相态对种子发芽率和可溶性蛋白质合成起抑制作用。其中甲醇相对发芽率抑制作用最大,抑制指数达到0.13;乙酸乙酯相对幼苗体内可溶性蛋白质合成抑制作用最大,抑制指数达到0.84。不同溶剂浸提薄壳山核桃叶获得的化感物质对高丹草种子萌发和幼苗生长影响不同,可能与其体内不同种类的蛋白质合成与表达变化有关。
To explore effects of allelochemicals in Carya illinoensis leaves on plant growth, with indoor seed germination method and bioassay, a study was made on effects of petroleum ether, chloroform, ethyl acetate and methanol extract liquids on the seed germination and seedling growth of Gaodan grass, a hybrid of Sorghum bicolor and Sorghum sudanense. The results showed that different solvent extracts showed different effects on the seed germination and growth of Gaodan grass. Methanol had highly significant inhibitory effects on the seed germination rates, germination potential, germination index and stem length with inhibition indexes reaching 0.13, 0.13, 0.23 and 0.61 respectively, and it had significant inhibitory effects on the fresh weight and root length of the seedlings with inhibition indexes reaching 0.13 and 0.31. Ethyl acetate also had extremely significant inhibitory effects on the seed germination indexes with the inhibition index reaching 0.13. Petroleum ether and chloroform had different effects on such indexes as the germination potential, mean germination speed, root length and stem length with no significant difference. All the solvent extracts had inhibitory effects on the seed germination rates and soluble protein synthesis with methanol having the most inhibitory effects on the seed germination rates and the inhibition index reached 0.13, and ethyl acetate having the most inhibitory effects on the soluble protein synthesis and the inhibition index reaching 0.84.Different solvent allelopathic extracts of Carya Illinoensis leaves had different effects on the seed germination and seedling growth of Gaodan grass and this may be related to changes of different protein synthesis and expression.
出处
《安徽林业科技》
2018年第1期24-28,共5页
Anhui Forestry Science and Technology
基金
安徽省教育厅自然科学研究重点项目(KJ2016A680)
黄山学院校地合作项目共同资助
