摘要
目的了解中国广东地区2010—2016年淋球菌对头孢曲松的敏感性以及相应菌株的淋球菌多抗原测序分型(NGMAST)基因型别。方法 2010—2016年在广东省疾病预防控制中心收集的285株淋球菌,经分离纯化及鉴定后,采用美国临床实验室标准化委员会(CLSI)推荐的琼脂稀释法测定其对头孢曲松的最小抑菌浓度(minimal inhibitory concentration,MIC);菌株培养后利用试剂盒提取DNA,并进行淋球菌多抗原测序分型(NG-MAST)。结果本次测试的285株淋球菌除1株MIC>0.25μg/mL外,其他都属于敏感菌株(CLSI敏感标准为≤0.25μg/mL),MIC≥0.06μg/mL的菌株比例为63.2%,其中2016年度MIC≥0.06μg/mL菌株比例为44.4%,2010年MIC≥0.06μg/mL菌株比例为70.6%。NG-MAST分型研究显示,285株淋球菌共有166个型别,菌株多样性较高,其中73种为已知型别,93种为新型别。测定的所有菌株中主要包括ST568(n=13),ST270(n=9),ST421(n=7),ST2288(n=5),ST1731(n=4),ST1766(n=4),ST1866(n=4),ST1870(n=4),ST1053(n=4),ST2318(n=4),ST5990(n=4),ST1614(n=4),ST1866(n=3)等。相同NG-MAST型别的菌株具有相同或相近的MIC值。结论广东地区淋球菌对头孢曲松MIC≥0.06μg/mL菌株比例较高,需要对此进行长期监测。7年间菌株的优势型别有较大变化,显示该地区性网络可能发生较大波动。NG-MAST分型可以作为分子生物学标记用于淋球菌耐药监测。
Objective To investigate the susceptibility to ceftriaxone and the prevalence of NG-MAST genotypes of Neisseria gonorrhoeae in Guangdong, China. To explore the correlation between MIC and NG-MAST genotypes. Methods A total of 285 N. gonorrhoeae isolates were collected from 2010 to 2016 in Guangdong, China. The susceptibility of the isolates to ceftriaxone were tested using the agar dilution method recommended by CLSI. DNA was extracted by Qiagen commercial kit and NG-MAST was performed using the molecular typing method. Results All isolates were susceptible to ceftriaxone （CLSI standard： S≤0.25μg/mL） except one isolate （MIC 0.5μg/mL）. The proportion of Neisseria gonorrhoeae with MIC≥0.06μg/mL was 63.2%. The proportion of isolates with MIC ≥0.06μg/mL in 2010 and 2016 was 70.6% and 44.4% respectively. A total of 166 genotypes were identified and 93 genotypes were new. The prevalent cluster included ST 568 （n=13）, ST270 （n=9）, ST421 （n=7）, ST2288 （n=5）, ST1731 （n=4）, ST1766 （n=4）, ST1866 （n=4）, ST1870 （n=4）, ST1053 （n=4）, ST2318 （n=4）, ST5990 （n=4）, ST1614 （n=4）, and ST1866 （n=3）. Isolates with identical or similar genotypes have similar MIC to ceftriaxone. Conclusion Ceftriaxone and spectinomycin were recommended to be used for Gonorrhoea treatment in Guangdong. The prevalent diversity of N. gonorrhoeae genotypes suggested a fluctuation in sexual network in this region. The correlation between genotypes and MIC suggested that NG-MAST can be used as a genetic marker in the antibiotic susceptibility surveillance system.
出处
《中国抗生素杂志》
CAS
CSCD
2018年第2期233-237,共5页
Chinese Journal of Antibiotics
