摘要
目的通过研究铜绿假单胞菌Ⅰ类整合子阳性株群体感应系统基因lasR与Ⅰ类整合子整合酶基因intI1表达水平的相关性,初步探讨群体感应系统对Ⅰ类整合子的调控机制。方法通过荧光定量RT-PCR方法检测铜绿假单胞菌群体QS信号分子受体基因lasR和Ⅰ类整合子整合酶基因intI1在液相菌与生物被膜菌中mRNA的表达水平,分析两基因表达的相关性。结果铜绿假单胞菌在生物被膜中群体感应系统lasR基因及Ⅰ类整合子intI1基因的mRNA表达水平高于液相菌,两者表达水平呈现正相关性(r=0.695,P<0.05)。结论铜绿假单胞菌群lasR和intI1基因表达呈正相关,提示intI1基因表达可能与群体感应系统的调控作用有关。
Objective The mechanism of the regulation of the quorum-sensing system on the class 1 integron was preliminarily explored by studying the expression correlation between the quorum-sensing system lasR gene and the class 1 integron integrase intI1 gene of Pseudomonas aeruginosa. Methods Fluorescence quantitative RT- PCR was used for detecting the mRNA expression level of the quorum-sensing system signal molecular receptor lasR gene and the class 1 integron integrase intI1 gene in the planktonic bacteria and biofilm bacteria of Pseudomonas aeruginosa. The correlation of the two gene expression was then analyzed. Results In biofilm, the mRNA expression level of Pseudomonas aeruginosa quorum-sensing system lasR and the class 1 integron intI1 gene was greater than that in planktonic bacteria, and both expression levels had a positive correlation （r=0.695, P〈0.05）. Conclusion The expression of the lasR and the intI1 gene of Pseudomonas aeruginosa have a positive correlation, which suggests that the intI1 gene expression may be related to the regulation of the functions of quorum-sensing system.
出处
《中国抗生素杂志》
CAS
CSCD
2018年第2期228-232,共5页
Chinese Journal of Antibiotics
基金
重庆市集成示范计划项目(No.cstc2015jcsf10002)
