摘要
采用索氏提取法,以正己烷为溶剂,提取金银忍冬鲜叶和鲜花中的活性物质,通过气相色谱-质谱(GC-MS)法分析其挥发性化学成分并通过抑菌活性实验分析其抑菌活性。分析结果表明:金银忍冬鲜叶提取物中GC含量最高的化合物是(-)-异长叶醇(22.63%),其次是2,7,10-三甲基-十二烷(10.48%)。金银忍冬鲜花提取物中GC含量最高的化合物是2,7,10-三甲基-十二烷(14.16%),其次是邻苯二甲酸二丁酯(9.60%)。金银忍冬鲜叶提取物质量浓度为0.19 g/m L时,对金黄色葡萄球菌的抑制作用最明显,抑菌圈直径达到13.81±1.48 mm;金银忍冬鲜花提取物在质量浓度0.027 5~0.22 g/m L范围内,对大肠杆菌有较好的抑制作用。根据半数抑制浓度比较提取物对3种供试菌种的抑制能力,结果显示金银忍冬鲜叶及其鲜花提取物对3种实验菌的抑制效果顺序均为金黄色葡萄球菌>大肠杆菌>枯草芽孢杆菌。
Active substance of Lonicera maackii（Rupr.） Maxim. fresh leaves and fresh flowers were respectively extracted by Soxhlet extraction method with hexane as solvent. The volatile components were analyzed by gas chromatography-mass spectrometry（ GC-MS）, and their antibacterial activities were analyzed by antibacterial activity experiment. The results showed that the highest GC content compound was （-）-isolongifoh）l （22.63%）, the second was 2,7,10-trimethyl-dodeeane （ 10.48 % ） in the fresh leaves extracts of Lonicera maackii. The highest GC content compound was 2,7,10-trimethyl-dodeeane （ 14.16 % ） , and the second was the dibutyl phthalate（9.60%） in the fresh flowers extracts of Lonicera maackii. When the mass concentration of fresh leaves extracts of Lonicera maackii was 0.19 g/mL, the inhibitory effect on Staphylococcus aureus was the highest with the antibacterial circle diameter of 13.81±1.48 mm. Lonicera maackii（ Rupr. ） Maxim. fresh flowers extract had good inhibition effect on Escherichia coli in the mass eoncenlration of 0.027 5- 0.22 g/mL range. Aeeording to the half inhibitory concentration of Lonicera maackii（Rupr.） Maxim. extract on three kinds of test strains, the results showed that Lonicera maackii（Rupr.） Maxim. fresh leaves extract aud its flower extract of three kinds of experimental bacteria followed the inhibitory sequence of Staphylococcus aureus〉 Escherichia coli 〉 Bacillus subtilis.
出处
《生物质化学工程》
CAS
北大核心
2018年第1期10-16,共7页
Biomass Chemical Engineering
基金
黑龙江省自然科学基金资助项目(C201411)
技术开发项目企业横向课题(07043215003)
关键词
金银忍冬提取物
GC-MS
抑菌活性
Lonicera maackii extracts
gas chromatography-lnass spectrometry （GC-MS）
antibacterial activity
