摘要
【目的】探讨2种不同成熟度杨桃(黄熟杨桃和绿熟杨桃)对橘小实蝇雌虫产卵量的影响并分析2者挥发物成分的差异。【方法】采用产卵杯法比较分析黄熟杨桃和绿熟杨桃对橘小实蝇雌虫产卵量的差异,同时采用固相微萃取技术(solid phase microextraction,SPME)和气相色谱-质谱联用技术(GC-MS)对2者的挥发性化学成分进行种类和含量的比较分析。【结果】黄熟杨桃对橘小实蝇雌虫的产卵量均显著高于绿熟杨桃。2种不同成熟度杨桃的挥发性物质在种类和含量上存在明显差异,黄熟杨桃中共检测出30种气味挥发性物质,而绿熟杨桃中共检测出24种气味物质,且有多种化合物在黄熟杨桃中存在而在绿熟杨桃中未检测出。除此之外,黄熟杨桃中的一些物质,如己酸甲酯、乙酸-反-2-己烯酯、庚酸甲酯、罗勒烯异构体混合物、辛酸甲酯、β-紫罗酮和4,6(Z),8(Z)-大柱三烯这7种物质的含量均明显高于绿熟杨桃。【结论】己酸甲酯、乙酸-反-2-己烯酯、庚酸甲酯、罗勒烯异构体混合物、辛酸甲酯、4,6(Z),8(Z)-大柱三烯、β-紫罗酮这7种物质可能是黄熟杨桃引诱橘小实蝇产卵的活性物质。
[Objective] Bactrocera dorsalis （Hendel） （Tephritidae： Diptera） is a major pest of fruit crops. It is highly polyphagous and causes economic losses to a wide range of fruit crops, consisting of more than 250 species of fruit and vegetables. The insect has a strong reproduction capability, with each female able to produce 400-1 800 eggs. Female B. dorsalis lay their eggs in mature fruits, while they are still on the tree, which causes the fruit to rot and fall in advance. Thus, oviposition is the main cause of serious harm to fruits and vegetables by the female B. dorsalis. Therefore, oviposition from the female B. dorsalis will be the key entry point to control any potential harm from B. dorsalis. At present, the control method to use against B. dorsalis is primarily induced and chemical control. However, B. dorsalis have developed a strong resistanance to a variety of chemical pesticides. Therefore, the control effect of chemical pesticides is greatly reduced. Pesticides can seriously affect environmental safety. But the effective attractant, methyl eugenol, is primarily used to lure and control the male B. dorsalis. It has been reported that methyl euge- nol is not only a safety hazard to the ecological balance, but also may induce liver cancer in mammals. Therefore, the search for a new attractant in the host fruit will be an effective way to control B. dorsalis.The attractive effects of various host fruit volatiles on the female flies have been reported. Starfruit is one of the important hosts of B. dorsalis. There previously has been no report on the research of the oviposition lure of starfruit to female B. dorsalis. In this paper, the effect of two different maturities of starfruit （yellow ripe starfruit and green ripe starfruit） on the oviposition of female B. dorsalis and the differences of their volatile components of yellow and green ripe starfruit were compared and analyzed using a solid phase mi- croextraction-gas chromatography-mass spectrometry （SPME-GC-MS）. It was also speculated
出处
《果树学报》
CAS
CSCD
北大核心
2018年第1期94-100,共7页
Journal of Fruit Science
基金
广东省省级科技计划项目(2014A020208129)
广州市科技计划项目(201607010090)
广东省自然科学基金项目(2015A030313601)
2017年省级大学生创新创业训练计划项目(201711347058)
