摘要
目的:研究4'-羟基汉黄芩素(4'-hydroxywogonin)对急性淋巴细胞白血病细胞株SUP-B15和Jurkat细胞增殖、凋亡的影响,并探讨其作用机制。方法:体外培养SUP-B15和Jurkat细胞,经不同浓度的4'-羟基汉黄芩素处理细胞后,用CCK8法测定4'-羟基汉黄芩素对细胞增殖的抑制作用;Annexin V-APC/7-AAD双染流式细胞术检测细胞凋亡;Western blot检测BCL-2,cleaved caspase-3,C-MYC等蛋白表达情况。结果:4'-羟基汉黄芩素呈剂量依赖性抑制SUP-B15和Jurkat细胞的增殖(r=0.78,r=0.89),24 h IC_(50)分别为(6.32±0.53)μg/ml,(12.04±0.42)μg/ml。在梯度药物浓度下,细胞凋亡率增高。Westwrn blot检测结果显示,4'-羟基汉黄芩素使凋亡相关蛋白BCL-2表达下调(P<0.01);cleaved caspase-3表达上调(P<0.01);细胞增殖相关蛋白C-MYC表达下调(P<0.01)。结论:4'-羟基汉黄芩素可通过抑制SUP-B15和Jurkat细胞的增殖及诱导其凋亡而发挥抗肿瘤作用,其机制可能与下调BCL-2,C-MYC表达,上调cleaved caspase-3蛋白表达有关。
）Objective： To investigate the effect of 4＇-hydroxywogonin on proliferation and apoptosis of human acute lymphoblastic leukemia SUP-B15 and Jurkat cells,and to analyze its possible mechanism. Methods： SUP-B15 and Jurkat cells were cultivated in vitro and treated with different concentrations of 4＇-hydroxywogonin,the inhibitory effect of 4＇-hydroxywogonin on the proliferation of SUP-B15 and Jurkat cells was detected by CCK-8 method; the cell apoptosis was examined by the flowcytometry with Annexin V-APC/7-AAD donble staining; the expression of C-MYC,BCL-2 and cleaved caspase 3 in SUP-B15 and Jurkat cells were measured with Western blot. Results： 4＇-hydroxywogonin inhibited the proliferation of SUP-B15 and Jurkat cells in a dose-dependent manner（ r = 0. 78,r =0. 89）,with IC50 value of（ 6. 32 ± 0. 53） μg/ml in SUP-B15 cells and（ 12. 04 ± 0. 42） μg/ml in Jurkat cells at 24 h.The early apoptotic rate of cell was also enhanced with the increase of 4＇-hydroxywogonin concentrations. The results of Western blot showed that 4＇-hydroxywogonin could down-regulate the expression of proliferation-related molecule CMYC（ P〈0. 01） and apoptosis-related molecule BCL-2（ P〈0. 01）,the expression of apoptosis-related molecule cleaved caspase 3 was up-regulate（ P〈0. 01）. Conclusion： 4＇-hydroxywogonin shows the effects of anti-tumor by inducing cell apoptosis and inhibiting cell proliferation,its molecular mechanism maybe relate with down-regulation of CMYC and BCL-2 expression and up-regulation of the cleaved caspase 3 expression.
出处
《中国实验血液学杂志》
CAS
CSCD
北大核心
2018年第1期65-69,共5页
Journal of Experimental Hematology
