摘要
【目的】为了寻找与小麦三雌蕊基因Pis1连锁的分子标记。【方法】利用川麦28(CM28)与其三雌蕊近等基因系CM28TP杂交的F2群体为研究材料,采用SRAP分子标记技术,结合分组混合分析法(BSA)筛选与Pis1基因连锁的分子标记。【结果】(1)1936个SRAP引物组合在两亲本CM28和CM28TP中具有多态性的引物组合为224对,多态性引物比例为11.57%;(2)利用三雌蕊池和正常池进一步筛选得到m2e36和m16e26 2个与Pis1基因连锁的SRAP标记;(3)利用218个F2分离群体检测,算出m2e36与Pis1基因的图距为18.22 cM,m16e26与Pis1基因的距离为22.63 c M,这2个分子标记分别位于Pis1的两侧。【结论】该结果为进一步利用SRAP标记进行小麦基因定位奠定了基础。
【Objective】This paper aimed to identify the molecular markers linked to three pistils gene（ Pis1） in wheat.【Method】The F2 population produced by from the crossed of Chuanmai 28（ CM28） and its near isogentic line for three pistils CM28 TP were used as materials.The molecular marker associated with Pis1 was screened by bulked sergeant analysis（ BSA） method and SRAP technology. 【Result】（ i） 224 pairs showed polymorphism among 1936 pairs of primer,the polymorphic percentage was 11. 57 %;（ ii） Two specific SRAP marker,m2 e36 and m16 e26 were found link to Pis1 gene in three pistils and normal pistils DNA pool;（ iii） After further analysis the 218 F2 individuals found Pis1 gene located between the SRAP marker m2 e36 and m16 e26,18. 22 c M distant from m2 e36 and 22. 63 c M distant from m16 e26.【Conclusion】The results could offer the references for further mapping of wheat genes using SRAP markers.
作者
胡召杉
杨在君
彭正松
HU Zhao-shan;YANG Zai-jun;PENG Zheng-song(Key Laboratory of Southwest China Resource Conservation （ Ministry of Education） , China West Normal University, Sichuan Nanchong 637009, China;College of Agricultural Sciences, Xichang College, Sichuan Xichang 615013, China)
出处
《西南农业学报》
CSCD
北大核心
2017年第12期2629-2633,共5页
Southwest China Journal of Agricultural Sciences
基金
国家自然科学基金项目(31760425)
西华师范大学英才基金项目(17YC355)
