摘要
摘耍：通过比较10株鸭瘟强毒与11株鸭瘟弱毒的UL2基因序列（包括GenBank中所有登录的鸭瘟病毒UL2基因序列），发现鸭瘟弱毒的UL2基因均缺失了528bp的B片段。基于UL2基因B片段序列设计引物，建立了理论上能特异检测鸭瘟强毒的荧光定量PCR和常规PCR检测方法（即对鸭瘟强毒的检测为阳性，对鸭瘟弱毒的检测为阴性），并对PCR产物进行了克隆和测序分析。结果显示，2种检测方法除了对鸭瘟强毒的检测为阳性外，对5株鸭瘟弱毒的检测也均为阳性，PCR产物的测序分析也说明扩增产物为UL2基因的B片段。结果表明，不能通过在UL2基因的B片段设计引物，来建立特异检测鸭疸强毒的检测方法。
The UL2 genes of 10 duck plague virus （DPV） virulent strains, were compared with 11 DPV attenuated strains （include all the UL2 gene sequence on GenBank）. There is 528 bp （B frag- ment） deletion in the UL2 gene of DPV attenuated strain. A real-time quantitative PCR and a PCR were developed according to design the primers based on the B fragment of UL2 gene, and these detection methods were expected to specifically amplify DPV virulent strain. PCR products were cloned and sequenced. The results showed that these developed real-time quantitative PCR and PCR could not specifically amplify DPV virulent strain, and also amplify DPV attenuated strain. Sequence analysis confirmed that PCR product was the B fragment of UL2 gene. The results indi- cated that,it is not workable to develop a detection method specific for DPV virulent strain based on B fragment of UL2 gene.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2018年第2期272-275,共4页
Chinese Journal of Veterinary Science
基金
广西科技资助项目(桂科攻10100014-5)
国家”万人计划”领军人才专项资助项目(2016-37)
广西特聘专家专项经费资助项目(2011B020)
关键词
鸭瘟病毒
强毒IB片段
UL2基圆
duck plague virus
virulent strain
B fragment
UL2 gene
