摘要
根据文献报道的鸭瘟病毒基因序列,设计合成了一对引物,其扩增跨幅为498 bp。用这对引物,以采用纯水直接研磨临床病料组织再按常规酚-氯仿抽提 DNA 的方法制备模板,对2株鸭瘟病毒进行扩增,均获得了与设计大小相符的明亮条带,为阳性;而对其他7株非鸭瘟病毒病料的基因扩增不出任何条带,为阴性;以该 PCR 方法检测14份 DPV 临床病料,均为阳性,与病毒分离和血清中和试验的结果一致。敏感性试验表明可检测到10fg 的鸭瘟病毒 DNA 模板。研究建立的直接从临床样品极微量检测 DPV 的 PCR 方法,能达到鸭瘟病毒基因的快速诊断要求。
one pair of primers was designed according to the published sequences of duck plague virus(DPV).With the template prepared from the tissue of the infected ducks,two samples of positive DPV infection could amplify the special fragment of DPV,the length of 498bp,while seven samples of negative -duck plague virus and bacteria eouldnt gain the special fragment of DPV.With the method we desigued,Fourteen clinical samples of dead ducks were de- tected,and all of them were positive of DPV infection.The result showed that it was the same consequence as the methods of virus isolation and neutralization test.
出处
《黑龙江畜牧兽医》
CAS
北大核心
2004年第11期10-12,共3页
Heilongjiang Animal Science And veterinary Medicine
基金
四川省教育厅青年科技基金(01LA09)
四川省重点建设学科项目(SZD0418)
