摘要
目的：对镉染毒（Cd）大鼠给予小补肾汤,探讨小补肾汤对镉细胞免疫功能和氧化应激损伤的影响。方法：选用SPF级Wistar大鼠60只,随机分为正常组、模型组、小补肾汤干预组（高、中、低剂量）、乙酰半胱氨酸组。末次给药24 h后处死大鼠,计算胸脾指数。采用MTT法检测T淋巴细胞的转化能力,比色分析法测定大鼠血清SOD活性和MDA含量,ELISA法测定大鼠血清IL-2和TGF-β_1的含量,免疫组化法检测大鼠Bcl-2和Bax的表达。结果：与正常组比较,模型组大鼠的体质量明显减轻,胸脾指数及T淋巴细胞的刺激指数明显降低,SOD活性明显降低,IL-2含量明显降低,Bcl-2的蛋白表达明显降低,MDA含量和TGF-β_1的含量均明显升高,Bax的蛋白表达明显升高（P〈0.05或P〈0.01）。与模型组比较,小补肾汤中、高剂量组体质量及T淋巴细胞的刺激指数明显增高,Bcl-2的蛋白表达明显升高,高剂量组胸腺指数明显增高,各干预组脾脏指数、SOD活性、IL-2含量明显增高;而小补肾汤中、高剂量组Bax的蛋白表达明显降低,各干预组MDA含量和TGF-β_1的含量明显降低（P〈0.05或P〈0.01）。结论：小补肾汤对镉所致大鼠的免疫损伤和细胞凋亡具有保护作用。
Objective ： To study the effects of Dunhuang Prescriptions Small Kidney - Tonifying Decoction on the damage of cell immune function and oxidative stress in rats induced by exposure to cadmium. Methods ： Sixty SPF Wistar rats were randomized divided into six groups ： normal control group, cadmium - exposed group, four administrated groups. After killing, indexes of thymus and spleen were calculated by weighing method. The transformation ability of T lymphocyte was measured by MTT. The levels of SOD and MDA were detected by colorimetric assay. The contents of IL - 2 and TGF - 1β were analyzed by ELISA. The protein expressions of Bcl - 2 and Bax were tested by immunohistochemical staining. Results ： Compared with the normal control group, the weight of cadmium - exposed group was decreased significantly and the SOD activity was decreased significantly, while the MDA level of cadmium - exposed group was increased significantly （ P 〈0.01 ）. The thymus and spleen indexes of the cadmium - exposed group were lower than those in the normal control group（ P 〈 0.01 ）. The IL- 2 content of cadmium -exposed group was lower than those in the normal control group, but the TGF - 1β content of cadmium - exposed group was higher than those in the normal control group （P 〈 0.01 ）. The Bcl - 2 protein expression of cadmium - exposed group was lower than those in the normal control group, but the Bax protein expression of cadmium - exposed group was higher than those in the normal control group （ P 〈 0.01 ）. Compared with the cadmium - exposed group, the weights and the T lymphocyte stimulation indexs of middle, high dose Small Kidney -Tonifying Decoction groups were increased significantly （P 〈 0.05 or P 〈 0.01 ）. The spleen indexes of Small Kidney- Tonifying Decoction treated groups were higher than those in the cadmium - exposed group（P 〈0.05 or P 〈0.01 ）. Sim- ultaneously, the thymus index of high dose Small Kidney - Tonifying Decoction group was higher than those in the cadmi- u
作者
安方玉
颜春鲁
刘永琦
伍志伟
苏韫
薛娜
雒志义
朱俊燚
李广强
AN Fangyu;YAN Chunlu;LIU Yongqi;WU Zhiwei;Su Yunl;XUE Na;LUO Zhiyi;ZHU Junyi;LI Guangqiang(Provincial - Level Key Laboratory for Molecular Medicine of Major Diseases and The Study on Prevention and Treatment of Traditional Chinese Medicine, Gansu University of Traditional Chinese Medicine, Lanzhou 730000, Gansu, China;Key Laboratory of Dunhuang Medicine and Transformatian of Ministry of Education and Gansu Province,Lanzhou 730000, Gansu,China;Prevention College of Gansu University of Traditional Chinese Medicine, Lanzhou 73000, Gansu, China)
出处
《中华中医药学刊》
CAS
北大核心
2018年第2期438-441,I0023,I0024,共6页
Chinese Archives of Traditional Chinese Medicine
基金
甘肃省自然科学基金项目(1508RJZA046)
敦煌医学与转化省部共建教育部重点实验室开放基金项目(DHYX1213-004)
甘肃中医药大学中青年基金项目(ZQ2014-9)
关键词
镉染毒
小补肾汤
免疫细胞增殖
凋亡
cadmium infected
Small Kidney - Tonifying Decoction
immunocyte proliferation
apoptosis
