摘要
目的:探索BANCR对人肝癌细胞HepG2的增殖、凋亡、侵袭和血管新生的影响。方法:实时定量PCR(qRTPCR)检测BANCR的表达。BANCR siRNA和Scramble分别转染HepG2细胞,利用CCK-8检测细胞增殖情况,流式细胞术分析细胞凋亡,Transwell测试细胞侵袭能力,管腔形成实验分析血管新生能力,免疫印迹分析增殖细胞核抗原(PCNA)、Caspase-3和基质金属蛋白酶9(MMP-9),血管内皮细胞生长因子VEGF、酸性成纤维细胞生长因子b FGF和干扰素IFN-γ的蛋白表达。结果:肝癌细胞(HepG2)中BANCR的表达高于正常干细胞L02(P<0.05)。与对照组相比,BANCR siRNA组的细胞增殖倍数明显降低,且BANCR siRNA组具有更高的细胞凋亡率和较低的侵袭细胞数(P<0.05)。免疫印迹结果显示,与对照组相比,BANCR siRNA组细胞凋亡标记蛋白Caspase-3和IFN-γ的表达明显上升(P<0.05),细胞增殖和侵袭标记蛋白PCNA、MMP-9、Fn、Vimentin以及VEGF、b FGF的表达都明显受到抑制(P<0.05)。结论:siRNA干扰BANCR后大大促进人肝癌细胞HepG2的凋亡,严重抑制了细胞增殖、侵袭以及血管新生。
Objective： To analyze the effects of BANCR on proliferation,apoptosis,invasion and angiogenesis in human hepatocarcinoma cell line HepG2. Methods： The expression of BANCR was detected by quantitative real-time reverse transcription PCR（ qRT-PCR）. BANCR siRNA and Scramble was respectively transfected into human hepatocarcinoma cell line HepG2. Cell proliferation was detected by CCK-8. Flow cytometry was performed to analyze the apoptosis. Transwell assay was used to test the invasion. Angiogenesis was analyse by tube formation assay. Western blot was executed to check the expression of proliferating cell nuclear antigen（ PCNA）,caspase-3,matrix metalloprotein 9（ MMP-9）,vascular endothelial growth factor（ VEGF）,acidic fibroblast growth factor（ b FGF） and interferon-γ（ IFN-γ）. Results： The expression of BANCR in HepG2 was higher than L02（ P〈0. 05）. Compared with control group,the cell proliferation folds in BANCR siRNA was largely decreased. Besides,BANCR siRNA group had a higher apoptosis rate and less invasive cells（ P〈0. 05）. Western blot showed that the expression level of caspase-3 and IFN-γ was obviously enhanced in BANCR siRNA group,and the expression of PCNA,MMP-9,Fn,Vimentin,VEGF and b FGF was distinctly surpressed in BANCR siRNA group compared to control group（ P〈0. 05）. Conclusion： siRNA interference of BANCR promotes apoptosis and represses proliferation,invasion and angiogenesis in human hepatocarcinoma cell line HepG2.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2018年第1期25-30,共6页
Chinese Journal of Immunology
基金
国家自然科学基金(No.31301135)
河南省自然科学基金(No.162300410211)
河南省高等学校重点科研项目(No.18B310019)
河南省医学科技攻关计划项目(No.201203068)
