摘要
目的:检测长链非编码RNA BANCR在胃癌组织中的表达,并探讨其对胃癌细胞迁移、侵袭生物学行为的调控作用与相关机制。方法:采用荧光定量PCR(QPCR)和TCGA-STDA数据库分析胃癌组织和癌旁组织中BANCR的表达水平,采用小干扰RNA(siRNA)技术敲低胃癌细胞MKN28 BANCR的表达,采用Transwell、QPCR检测MKN28细胞的迁移、侵袭情况以及肿瘤细胞转移相关标志物的表达水平变化。结果:胃癌组织中BANCR的表达水平呈明显上调。敲低BANCR表达后,MKN28细胞的迁移、侵袭能力被抑制,转移相关分子CDH1的表达量显著增加,而Vimentin的表达量显著降低。此外,敲低BANCR下调ZEB1表达,同时干扰miR-203能部分恢复ZEB1的表达水平。结论:BANCR在胃癌组织中呈高表达,其可能通过调节miR-203-ZEB1-CDH1轴促进胃癌细胞迁移和侵袭。
Objective:To investigate the expression of lncRNA BANCR in gastric cancer(GC),and explore the potential biological function and underlying molecular mechanism on cell migration and invasion. Methods:The mRNA expression level of BANCR in GC tissues was detected by quantitative real-time PCR(QPCR). MKN28 cells was transfected with siRNA against BANCR. Transwell assays was used to detect the ability of cell migration and invasion and QPCR was used to detect the expression of metastasis-related markers. Results:The expression level of BANCR in GC tissues was significantly higher than that in adjacent tissues,which consistent with TCGA data. Down-regulation of BANCR repressed GC cell migration and invasion. Furthermore,the expression level of CDH1 was up-regulated,and the expression level of Vimentin and ZEB1 was down-regulated in BANCR knocking-down cells. Interesting,the expression level of ZEB1 was restored by decreasing miR-203 in BANCR knocking-down cells. Conclusion:BANCR was over-expressed in GC tissues and play an oncogenic role by regulating the miR-203-ZEB1-CDH1 axis in GC.
作者
崔鹤
居晓斌
叶琴
潘猛
周惠英
Cui He;Ju Xiaobin;Ye Qin;Pan Meng;Zhou Huiying(Department of Genetics Laboratory,the First Affiliated Hospital of NMU,Nanjing 210029,China)
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2018年第12期1674-1678,共5页
Journal of Nanjing Medical University(Natural Sciences)
基金
国家自然科学基金青年科学基金(81702789)
