摘要
目的探讨腹泻型感染后肠易激综合征(PI-IBS)患者外周血和肠黏膜细胞因子表达变化及其与临床症状积分的关系。方法选择2013年1-12月海南省人民医院门诊及住院的腹泻型PI-IBS患者30例(观察组),同期健康体检者30例(对照组)。分离培养两组对象的外周血单个核细胞(PBMC),ELISA检测外周血及细胞培养上清液中干扰素γ(IFN-γ)、白细胞介素(IL)-10水平;结肠镜取结肠黏膜组织,免疫组织化学检测结肠黏膜IFN-γ及IL-10蛋白表达。Spearman相关分析IFN-γ、IL-10水平变化与临床症状积分的相关性。结果两组对象外周血中IL-10、IFN-γ水平比较,差异无统计学意义(P>0.05);与对照组比较,观察组患者PBMC分离培养液中IFN-γ水平升高,IL-10水平降低,差异有统计学意义(P<0.01)。观察组患者肠道主要症状积分与PBMC培养上清液及结肠黏膜中IFN-γ表达水平呈正相关(r=0.45、0.94,P<0.01),与IL-10表达水平呈负相关(r=-0.52、-0.79,P<0.01)。结论 IFN-γ/IL-10失衡可能参与腹泻型PI-IBS发病,并可作为疾病活动的观察指标。
Objective To investigate the expression change of cytokines in peripheral blood and intestinal mucosa in the patients with diarrhea post-infectious irritable bowel syndrome（PI-IBS）and its relation with clinical symptoms scores.MethodsThirty outpatients and inpatients with diarrhea PI-IBS（observation group）and contemporaneous 30 individuals undergoing physical examination（control group）in the Hainan Provincial People′s Hospital from January to December 2013 were selected.The peripheral blood mononuclear cells（PBMC）were separated and cultured.Then the levels of IFN-γand IL-10 in peripheral blood and cell culture supernatant fluid were detected by ELISA.The colonic mucosal tissue was taken by coloscopy.Then colonic mucosal IFN-γand IL-10 protein expression was detected by immunohistochemistry staining.Furthermore,the correlationship between the level change of IFN-γand IL-10 with clinical symptom score was analyzed by using the Spearman correlation method.Results Peripheral blod IL-10 and IFN-γlevels had no statistical difference between the two groups（P〈0.05）.Compared with the control group,in PBMC seperation and cuture,the IFN-γlevel in the observation group was increased and IL-10 level was decreased,the difference was statistically significant（P〈0.01）.The intestinal main symptom score in the observation group had the positive correlation with IFN-γexpression level of PBMC culture supernatant fluid and colonic mucosal IFN-γexpression level（r=0.45,0.94,P〈0.01）,and had the negative correlation with IL-10 expression level（r=-0.52,-0.79,P〈0.01）.Conclusion The unbalance of IFN-γand IL-10 level could be involved in the pathogenesis of diarrhea PI-IBS,which can serve as the observation indicators of disease activity.
出处
《重庆医学》
CAS
2018年第4期433-435,共3页
Chongqing medicine
基金
国家自然科学基金资助项目(81160057)
海南省国际科技合作专项基金资助项目(KJHZ2013-14)
