摘要
研究鹿茸多肽(pilose antler peptide)对微波辐射后小鼠学习记忆的影响,并探讨其作用机制。采用2 450MHz、平均功率密度为10.0 m W/cm2微波照射小鼠90 min/d,连续28 d,建立小鼠微波辐射致学习记忆障碍模型。同时,微波照射后皮下注射鹿茸多肽25、50、100 mg/kg,连续给药28 d,29 d始采用Y迷宫实验检测小鼠的学习记忆能力,34 d Y迷宫实验结束后断头处死小鼠,酶免法检测脑组织中天冬氨酸(Asp)、γ-氨基丁酸(GABA)、谷氨酸(Glu)、S100B的水平,酶免法检测脑组织中Na^+-K^+-ATP酶、单胺氧化酶-B(MAO-B)的活性,黄嘌呤氧化酶法检测脑组织中超氧化物歧化酶(SOD)活性,酶免法检测脑组织中的肿瘤坏死因子α(TNF-α)含量,并取脑组织做HE(苏木精-伊红染色法)染色,光镜下观察脑海马齿状回区组织结构的形态学变化。结果表明,与对照组相比,照射组小鼠在Y迷宫实验中错误逃逸次数增加,脑组织中Asp、Glu含量明显降低、GABA含量明显增高,脑组织中MAO-B活性增高而Na^+-K^+-ATP酶、SOD活性降低,脑组织中S100B、TNF-α含量增高;与照射组比较,鹿茸多肽50、100 mg/kg减少Y迷宫错误逃逸次数;鹿茸多肽25、50、100 mg/kg增加脑组织中Asp及Glu含量,降低MAO-B活性;鹿茸多肽50、100 mg/kg降低脑组织中GABA、S100B、TNF-α含量,增强脑组织中SOD、Na^+-K^+-ATP酶活性(p<0.05或p<0.01)。病理可见照射组海马齿状回(DG)结构部分细胞体积变大,胞浆疏松、淡染,部分细胞核固缩状态;鹿茸多肽100 mg/kg组较照射组有所改善。实验证明鹿茸多肽(50、100mg/kg,连用28 d)对微波辐射所致小鼠的学习记忆障碍有一定的改善作用,机制可能与鹿茸多肽通过抗炎作用而减弱氧化应激、改善神经递质水平有关。
To investigate the effects of pilose antler peptide on learning and memory abilities of mice exposed to microwave radiation and to study its mechanism,learning and memory impairment model of mice was induced by microwave radiation of 2 450 MHz average surface power,10. 0 m W/cm2 for 28 d. The irradiated rats were treated with 25,50,and 100 mg/kg pilose antler peptide by subcutaneous injection for 28 d. The learning and memory ability of mice was tested by Y maze experiment from 29 d to 33 d. After Y maze experiment,all mice were decapitated on 34 th d,the contents of aspartic acid（Asp） 、γ-aminobutyric acid（GABA）,glutamic acid（Glu） and S100 B,the activities of Na~＋-K~＋-ATPase,monoamine oxidase-B（MAO-B）,level of tumor necrosis factor-α（TNF-α） in the brain of were determined with ELISA,respectively. The activity of superoxide dismutase（SOD） in the brain of mice was detected with xanthine oxidaseassay. The dentate gyrus area of hippocampal organizational structure was observed and analyzed by microscope after HE staining. The results indicated that compared with the control group,the irradiation group could increase the numbers of errors in Y maze experiment,and rise the contents of GABA,S100 B and TNF-α,lower the contents of Asp,Glu,increase the activity of MAO-B,lessen the activities of Na~＋-K~＋-ATPase,SOD in the brain of mice（p 0. 05 or p 0. 01）. Compared with the irradiation group,the pilose antler peptide（50,100 mg/kg） groups could significantly decrease the numbers of errors in Y maze experiment,and effectively reduce the contents of GABA,TNF-α and S100 B,improve the activities of Na~＋-K~＋-ATPase,SOD in the brain of mice. The Pilose antler peptide（25,50,and 100 mg/kg） groups could increase the content of Asp and Glu,lower the activity of MAO-B in the brain of mice（p 0. 05 or p 0. 01）. Pathology found that part of cell volume grew,cytoplasm was loosened and light dyed,part of the nucleus pycnosis was seen in the irradiation group.Compared with the irradi
作者
范红艳
王艳春
任旷
李曼
徐玉良
钟秀宏
刘师兵
吕士杰
FAN Hongyan;WANG Yanchun;REN Kuang;LI Man;XU Yuliang;ZHONG Xiuhong;LIU Shibing;LV Shijie(Jilin Medical University, Jilin Province Jilin City 132013)
出处
《辐射防护》
CAS
CSCD
北大核心
2018年第1期51-57,共7页
Radiation Protection
基金
吉林省卫生厅科技研究项目资助课题(No2014Z105
No2012Z060)
吉林省大学生创新创业训练计划项目(2015025)
关键词
鹿茸多肽
微波辐射
学习记忆
神经递质
TNF-Α
抗氧化
pilose antler peptide
microwave radiation
learning and memory
neurotransmitter
tumor necrosisfactor - α
antioxidation
