摘要
目的建立同时测定宝咳宁颗粒中柚皮苷、新橙皮苷、黄芩苷、汉黄芩苷质量分数的HPLC法。方法采用Dikma Spursil C18色谱柱(4.6 mm×250 mm,5μm);流动相为乙腈-质量浓度0.1%磷酸水溶液,梯度洗脱;流速为1.0 mL/min;柱温为30℃;检测波长为283 nm(柚皮苷、新橙皮苷)和275 nm(黄芩苷、汉黄芩苷);进样量为5μL。结果宝咳宁颗粒中4种黄酮苷类成分均呈良好的线性关系(r>0.999 0),平均加样回收率分别为95.7%(RSD为1.93%)、95.4%(RSD为2.10%)、103.5%(RSD为1.57%)、102.4%(RSD为2.50%)。结论方法准确可靠、重复性好,可用于宝咳宁颗粒的质量控制。
Objective To establish an HPLC method for simultaneous determination of naringin,neohesperidin, baicalin, and wogonoside in Baokening granules. Methods The analysis was performed on a Dikma Spursil C18 column（ 4. 6 mm × 250 mm,5 μm） with its temperature maintained at 30 ℃, mobile phase comprising of acetonitrile-0. 1% H3 PO4 with gradient elution at the flow rate of 1. 0 mL/min. The detection wavelengths were set at 283 nm and 275 nm. Results Four constituents showed good linear relationships（ r 〉0. 999 0）, whose average recoveries were 95. 7%（ RSD = 1. 93%）, 95. 4%（ RSD =2.10%）, 103. 5%（ RSD = 1. 57%）, and 102. 4%（ RSD = 2. 50%）, respectively. Conclusion The established method was proved to be accurate, reliable, and repeatable, which was suitable for the quality control of Baokening granules.
出处
《广东药科大学学报》
CAS
2017年第6期748-752,共5页
Journal of Guangdong Pharmaceutical University
基金
上海市药物(中药)代谢产物研究重点实验室开放课题(2015SHDX1001)
