摘要
目的探讨板蓝根水提液的纳滤浓缩工艺。方法采用高效液相色谱(HPLC)法测定(R,S)告依春保留率,检测波长为245 nm,流动相为甲醇-0.02%磷酸溶液(7∶93)。以(R,S)告依春保留率和膜通量为指标,通过单因素试验分析截留相对分子质量、操作压力、吸附特征等因素对板蓝根水提液浓缩工艺的影响。结果 (R,S)告依春的保留率随着膜相对分子质量减小而增大,受压力、浓缩倍数的影响较小;膜通量与压力、截留相对分子质量呈正相关,随着浓缩倍数的增大而减小;孔径200纳滤膜对(R,S)告依春的保留率为97.50%,膜通量60 L/(m^2·h)对板蓝根提取液中其他成分无明显影响,且浓缩效率高。结论纳滤浓缩适用于板蓝根的药液浓缩,效率高,成分损失少。
Objective To investigate the nanofiltration concentration process of aqueous extract of Radix Isatidis. Methods HPLC method was adopted to determine the retention rate of（ R,S） epigoitrin with mobile phase of methanol -0. 02% the phosphoric acid solution（ 7 ∶ 93）,the detection wavelength was 245 nm. With the retention rate and membrane flux of（ R,S） epigoitrin as the indexes,the effects of operating pressure,adsorption characteristics and other factors on concentration process were investigated by single factor tests. Results The retention rate of（ R,S） epigoitrin increased with decreasing of aperture and unchanged with change of pressure and cycles of concentratin. Membrane flux was positively correlated with intercept relative molecular weight and pressure,decreased as concentration ratioincreased. Retention rate of（ R,S） epigoitrin was 97. 50% when nanofiltration membranes aperture was 200 Da. When the membrane flux was 60 L/（ m^2·h）,the process had no obvious influence to other ingredients in Radix Isatidis with high concentration efficiency. Conclusion Nanofiltration concentration is suitable for concentrating liquid of Radix Isatidis,it has high efficiency and low losses of active ingredients.
作者
伍永富
朱河澄
冯天炯
罗维早
Wu Yongfu Zhu Hecheng Feng Tianjiong Luo Weizao(Taiji Group Chongqing No. 2 Chinese Medicine Factory Co. , LTD. , Chongqing, China 402284 Taiji Group Co. , LTD. , Chongqing , China 408000)
出处
《中国药业》
CAS
2017年第14期4-6,共3页
China Pharmaceuticals
关键词
(R
S)告依春
纳滤工艺
膜通量
保留率
（R
S） epigoitrin
nanofiltration process
membrane flux
retention rate
