摘要
目的:探讨电针对电刺激硬脑膜大鼠偏头痛模型5-HT_(1B)受体的调节作用。方法:选取成年雄性SD大鼠40只,随机分为5组,每组8只,对照组(C,只进行手术,不进行硬脑膜电刺激)、模型组(M,造模不给予电针)、单穴组(EA1,造模并给予电针风池穴)、双穴组(EA2,造模并给予电针风池穴、阳陵泉穴)和假穴组(SA,造模并给予电针假穴)。实验前检测面部机械痛阈基线,实验第2、4、6天分别测定大鼠足面部的机械痛阈。实验第7天取三叉神经脊束核、三叉神经核,用相对定量实时聚合酶链式反应测定5-HT_(1B)受体基因相对表达水平,用蛋白印迹法测定5-HT_(1B)受体蛋白相对表达水平。结果:与模型组比较,单穴组和双穴组的痛阈显著提高(P<0.05),且双穴组高于单穴组(P<0.05);单穴组和双穴组5-HT_(1B)受体基因表达和蛋白表达比模型组显著提高(P<0.05),且双穴组高于单穴组(P<0.05)。结论:电针对偏头痛大鼠模型有治疗作用,且双穴组优于单穴组。
Objective： To do research on the regulating effect of 5-HT_（1B）receptors in the migraine rat model of electrical stimulating dura mater via electroacupuncture. Methods： 40 male SD rats were divide into 5 groups,8 for each group. Except control group,the electrical stimulation of the dura surrounding the superior sagittal sinus was carried out at the 1 st,3 rd and 5 th day. The control group received electrode implantation surgery only. At the 2 nd,4 th and 6 th day,single acupoint group and double acupoint group were received electroacupuncture as treatment. Sham acupuncture group received acupuncture at a non-acupuncture point following dural stimulation. The facial mechanical withdrawal threshold was taken at the 2 nd,4 th and 6 th day following dural stimulation.Eight rats from each group were decapitated,and the TG and TNC were removed and detected 5-HT_（1B）receptor mRNA expression by real-time quantitative PCR. 5-HT_（1B）receptor protein was evaluated by western blot. Results： Compared with the control group,the mechanical withdrawal threshold of model group was declined significantly（ P〈0. 05）. Compared with the model group,the mechanical withdrawal threshold of single acupoint group and double acupoint group was declined significantly（ P〈0. 05）. Compared with the model group,5-HT_（1B） receptor mRNA and protein in TNC and TG tissue of rats in single control group was declined significantly（ P〈0. 05）. Compared with the model group,5-HT_（1B）receptor mRNA and protein in TNC and TG tissue of rats in single acupoint group and double acupoint group was increased significantly（ P〈0. 05）. Conclusion： The electroacupuncture could significantly improve the damage of rat dura mater of superior sagittal sinus in following electrical stimulation by releasing neurotransmitter,which may be the mechanisms of migraine treatment.
出处
《世界中医药》
CAS
2017年第12期3058-3062,共5页
World Chinese Medicine
基金
国家重点基础研究发展计划(973计划)项目(2014CB543203)
北京市医院管理局临床医学发展专项经费资助项目(ZYLX201412)
北京市自然科学基金资助项目(7154205)
