摘要
目的建立啤酒花中总黄酮和黄腐酚含量测定方法,对来自不同产地和品种的29个啤酒花样品中的总黄酮和黄腐酚进行测定。方法用芦丁-AlCl3分光光度法测定总黄酮的含量;用HPLC-UV法测定黄腐酚的含量。色谱条件:色谱柱为Dikma Technologies Diamonsil C18柱(250mm×4.6mm,5μm);流动相为乙腈-1%冰醋酸梯度洗脱,流速为1.0ml/min;柱温为25℃;检测波长为370nm。结果总黄酮回归方程为A=30.345C+0.016 8,r=0.999 9;黄腐酚回归方程为A=55 446C+9 040.5,r=0.999 9,表明总黄酮在20.2~404.0μg/ml,黄腐酚在2.152~43.040μg/ml范围内,线性关系良好,两者精密度和重复性RSD均<2%,平均加样回收率分别为102.71%和100.21%。结论不同种类的啤酒花之间,总黄酮和黄腐酚含量存在巨大差异,进口啤酒花优于国产。
Objective To determine the contents of total flavonoids and xanthohumol in hop from 29 different countries and regions.Methods Rutin colorimetric method was used to determine the content of total flavonoids.HPLC-UV method was established for the determination of xanthohumol in hops.HPLC method was performed by Dikma Technologies Diamonsil C18（250 mm×4.6 mm,5μm）column with mobile phase acetonitrile-1% glacial acetic acid solution at the flow rate of1.0 ml/min.The column temperature was 25 ℃.The detection wavelength was 370 nm.Results The equation of linear regression of total flavonoids was A=30.345 C＋0.016 8,r=0.999 9.The equation of linear regression of xanthohumol was A=55 446 C＋9040.5,r=0.999 9.Their linear ranges were respectively 20.2-404.0μg/ml,2.152-43.040μg/ml,which indicated a good linear relationship.The RSDs of precision and repeatability were less than 2%.The average recoveries of flavonoids and xanthohumol were respectively 102.71% and 100.21%.Conclusion The contents of total flavonoids and xanthohumol in different hops varieties are significantly different and the import hops was better than the domestic hops in this study.
作者
李常惠
许月明
张巧艳
韩婷
辛海量
LI Changhui;XU Yueming;ZHANG Qiaoyan;HAN Ting;XIN Hailiang(School of Pharmacy , Shandong Univer-sity of Traditional Chinese Medicine , Jinan 250355 , C h i n a;Sch o o l of P h a r m a c y , Jiamusi University , Jiamusi 1 5 4 0 0 7 ,C h i n a;School of Pharmacy , Second Military Med i c a l University , S h a n g h a i 2 0 0 4 3 3 , C h i n a)
出处
《药学实践杂志》
CAS
2018年第1期64-67,共4页
Journal of Pharmaceutical Practice
基金
国家自然科学基金(U1603283)
关键词
啤酒花
总黄酮
黄腐酚
Humulus lupulus L .
total flavonoids
xanthohumol
