摘要
目的：建立大肠埃希菌（Escherichia coli）感染哺乳期西藏小型猪腹泻模型,研究葛根芩连汤（GQT）治疗婴幼儿E.coli感染性腹泻作用机制。方法：按照体重灌胃不同剂量的E.coli,灌胃后每3 h进行体重,体温,和腹泻状况的观察并收集粪便进行E.coli计数;分别设置阳性药蒙脱石散组（93.5 mg·kg^-1）,GQT高、中、低剂量组（含生药12.1,6.05,1.21 g·kg^-1）,给药3 d,每天2次。每次给药后观察腹泻指数、体重变化,统计生存率,检测粪便中E.coli菌落数,血液白细胞数（WBC）,酶联免疫吸附法（ELISA）检测血清肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）含量。结果：E.coli剂量为1×109CFU·kg^（-1）时,6 h仍为水样泻,粪便E.coli计数〉1×1011CFU·g-1,无明显自愈倾向;GQT低剂量组可显著抑制腹泻,中、高剂量组无明显止泻作用;高、中、低剂量组可显著降低粪便E.coli和血液WBC的数量,抑制血清TNF-α和IL-6的表达。结论：当E.coli灌服剂量为1×109CFU·kg^（-1）时,6 h仍为水样泻,且粪便E.coli计数〉1×1011CFU·g-1,则判定造模成功;GQT可通过抗炎和抑制E.coli的增殖减轻E.coli感染哺乳期小型猪腹泻。其抗炎作用可能与减少炎症因子TNF-α和IL-6有关。
Objective： To establish the Escherichia coli-infected diarrhea model of suckling Tibetan minipigs,in order to study the mechanism of Gegen Qinlian Tang（GQT） on E. coli-infected diarrhea in infants.Method： The mini-pig diarrhea model was established through oral administration with pathogenic E. coli. The animals were divided into control group（Montmorillonite powder,93. 5 mg·kg^-1）,low-dose group（1. 21 g·kg^-1）,middle-dose group（6. 05 g·kg^-1） and high-dose group（12. 1 g·kg^-1）. Fecal E. coli count and white blood cell count were detected after each administration,and the serum tumor necrosis factor-α（TNF-α） and interleukin-6（IL-6） were detected by enzyme linked immunosorbent assay（ELISA） kit. The diarrhea index,weight change and survival rate were observed. Result： When the dose of E. coli was 1 × 109 CFU·kg^（-1）,the watery diarrhea was maintained at 6 h,the count of fecal coliform was higher than 1 × 1011 CFU·g-1,and there was no obvious trend of self-cure. High,medium and low-dose GQT groups showed significant reduction in fecal E. coli count and blood white blood count,and inhibition of the expressions of serum TNF-α and IL-6. Low-dose group showed significant inhibition of diarrhea,and middle and high-dose groups had no obvious antidiarrheal effect.Conclusion： When the dose of E. coli was 1 × 109 CFU·kg^（-1）,the feces were still watery at 6 h,and the E. coli count of fecal was higher than 1 × 1011 CFU·g-1,which proved that the E. coli-infected diarrhea mini-pig model was successfully established. GQT could relieve E. coli-infected diarrhea among suckling pigs by resisting inflammation and inhibiting the proliferation of E. coli. The anti-inflammatory effect may be related to the reduction of inflammatory factors TNF-α and IL-6.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2018年第3期125-131,共7页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81374049)
关键词
葛根芩连汤
大肠埃希菌腹泻
哺乳期小型猪
止泻
抗炎
Gegen Qinlian tang
Escherichia coli diarrhea
suckling mini-pig
antidiarrheal
anti-inflammation
