摘要
利用Illumina高通量测序技术对某养猪场不同处理单元细菌和真菌群落变化特征进行分析,并采用冗余性分析方法考察其受水质参数的影响。结果表明:活性污泥各阶段主要细菌类群为变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidetes)和放线菌门(Actinobacteria);生物处理后,变形菌门(Proteobacteria)的相对丰度降低近15%,厚壁菌门(Firmicutes)的相对丰度从进水到出水降低了近6%,同时放线菌门(Actinobacteria)的相对丰度增加近7%;主要真菌类群为子囊菌门(Ascomycoka)、担子菌门(Basidiomycota)和少量壶菌门(Chytridiomycota)。值得注意的是处理后出水中仍含有丰度较高的曲霉菌属(Aspergillus)、毛孢子菌属(Trichosporon)、念珠菌属(Cladosporium)等真菌致病菌,对后续环境和公共健康造成潜在威胁。养猪废水处理系统众多细菌和真菌群落的变化受水质参数(主要是COD和氮)的影响明显。
The characteristics of bacterial and fungal community changes in different treatment units of a pig farm was analyzed by Illumina high-throughput sequencing technologies. Their influence on those water quality parameters was inspected by means of redundancy analysis. The results show that the main bacterial groups in the activated sludge were Proteobacteria,Firmicutes,Bacteroidetes and Actinobacteria; After biological treatment,the content of Proteobacteria decreased by nearly 15%,while the one of Firmicutes dropped by nearly 6% from incoming water to out water,and in the meantime the content of Actinobacteria increased by almost 7%; the main fungi groups are Ascomycoka,Basidiomycota and a small amount of Chychomycetes. It 's worth noting that after treatment, the effluent still contained abundant fungal pathogens such as Aspergillus, Trichosporon and Cladosporium,which can be potential threats to future environmental and public health. The variation of many bacterial and fungal community in swine wastewater treatment system is significantly affected by water quality parameters( mainly COD and nitrogen concentration).
出处
《水资源保护》
CAS
CSCD
北大核心
2018年第1期88-94,共7页
Water Resources Protection
关键词
养猪废水
活性污泥
群落
致病菌
冗余性分析
swine wastewater
activated sludge
community
pathogenic bacteria
redundancy analysis
