摘要
基于对氯苯丙氨酸(PCPA)抑郁模型,将60只Sprague-Dawley(SD)大鼠随机分为对照组、模型组、氟西汀组和叶酸组(50mg/kg,75mg/kg),通过悬尾、强迫游泳实验和糖水消耗量实验评价大鼠抑郁行为,探究了叶酸的抗抑郁效果及其作用机制。采用高效液相色谱法(HPLC)测定大鼠体内血清皮质酮及脑组织中5-HT神经递质的含量。结果显示,PCPA模型大鼠情绪低落,不动时间明显增加(P<0.05);血清中皮质酮含量显著上升(P<0.05),联合皮质和海马脑区的5-HT含量均显著下降(P<0.05)。叶酸可以显著缓解以上变化(P<0.05或P<0.01),叶酸对PCPA模型的抗抑郁作用可能与降低大鼠血清皮质酮含量,进而提高前额叶联合皮质与海马5-HT水平有关。
The present study was to investigate the antidepressant function of Folic Acid in depressive rats induced by PCPA. Sixty male SD rats were divided into control group （CG）, model group （MG）, Fluoxetine treated-model group（FAG）, and folic acid treated-model group（FAG, 50 mg/kg and 75 mg/kg）. The changes of depressive behaviors were evaluated by tail suspension test, force swimming test and sugar consumption. The corticosterone in serum and 5-HT in brain were evaluated by HPLC. Results showed that folic acid can significantly decrease the immobility time in tail suspension test, force swimming test, obviously decrease the level of eorticosterone in serum and increase the levels of 5-HT in combined cortex and hippocampus（P〈0.05 or P〈 0.01）. Folic acid has antidepressant effects, and its mechanism may be related to reducing the corticosterone in serum, and improvements of 5-HT in combined cortex and hippocampus.
出处
《陕西师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2018年第1期80-83,共4页
Journal of Shaanxi Normal University：Natural Science Edition
基金
上海市科委科研基金(16DZ2348900)
国家大学生创新创业基金(201510269136
201610269085)
