摘要
本实验主要研究马齿苋提取物对抗湿疹瘙痒的作用机制。实验采用DNCB建立大鼠急性湿疹模型,然后经皮给予不同浓度的马齿苋提取物(20、10、5 g/kg),每天一次,连续10 d。末次给药技术后评价各实验组大鼠湿疹严重程度,测定皮肤TEWL,免疫组化法测定LTA4、SP、HT H1、IL-31、PAR2和TRPV1水平及分布,RTPCR测定皮肤Filaggrin基因表达,Western-blot测定Filaggrin蛋白表达。结果显示,马齿苋可显著减轻大鼠湿疹病变程度;减小大鼠TEWL水平;减少湿疹大鼠皮肤LTA4、SP、HT H1、IL-31、PAR2和TRPV1蛋白表达水平;增加大鼠皮肤细胞Filaggrin基因和蛋白表达。提示马齿苋可显著对抗大鼠皮肤湿疹病变,其机制可能与降低湿疹炎症因子和瘙痒相关因子表达以及上调皮肤屏障系统中Filaggrin表达有关。
The present study described the protective mechanism of purslane on acute eczema induced by DNCB in rats. Rats acute eczema model were established by DNCB ,then the eczema rats were treated with purslane extract （20,10,5 g/kg） transdermally once a day, for total 10 days. At the end of medication, rats eczema level was evaluated, skin TEWL was measured,levels and distributions of LTA4, SP, HT H1, IL-31, PAR2 and TRPV1 were detected by immunohisto- chemistry method,and Filaggrin gene and protein were detected by RT-PCR and Western-blot. The results showed that purslane can significantly reduce rats eczema scores,rats TEWL and the expression of LTA4, SP, HT H1, IL-31, PAR2 and TRPV1 proteins. In addition, Filaggrin gene and protein expression had been significantly increased. The experi- ment results implied that purslane can significantly protect against the lesions of eczema, which may be associated with decreasing expression of inflammatory cytokines and itching factors and regulate Filaggrin expression in skin barrier sys- tem.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2017年第12期2017-2023,共7页
Natural Product Research and Development
基金
国家自然科学基金(81403405)
关键词
马齿苋
急性湿疹
瘙痒
干预机制
purslane
acute eczema
itch
intervention mechanism
