摘要
目的:探讨乳腺肿瘤患者血清和组织多效蛋白(PTN)表达及其在乳腺肿瘤诊断和预后评估中的价值。方法:选择120例乳腺肿瘤患者(观察组)和100例健康人群(对照组)作为研究对象,采集各组受试对象静脉血3ml,采用酶联免疫吸附试验检测血清PTN含量,同时用化学发光免疫分析法检测血清CA15-3和CEA含量,利用受试者工作曲线(ROC)研究血清PTN对乳腺肿瘤的诊断价值。采用免疫组织化学染色检测观察组肿瘤组织及肿瘤旁组织中PTN表达水平,按照乳腺肿瘤组织PTN表达水平将乳腺肿瘤患者分为PTN高表达组和低表达组,分析PTN表达水平与患者生存预后的关系。结果:观察组血清PTN含量明显高于对照组,2组间比较差异有统计学意义(P<0.05)。在最佳截断值时,PTN诊断乳腺肿瘤的曲线下面积(AUC)为0.947(95%CI:0.916~0.978,P<0.05),血清PTN诊断乳腺肿瘤的AUC高于血清CA15-3、CEA。乳腺肿瘤组织PTN表达评分为7.15±0.49分,肿瘤旁组织PTN表达评分为2.04±0.12分,肿瘤组织PTN表达明显高于肿瘤旁组织(P<0.05);PTN低表达组术后生存时间明显高于PTN高表达组,2组间比较差异有统计学意义(χ2=4.003,P=0.038)。PTN表达与肿瘤直径、淋巴结转移、雌激素受体(ER)表达、孕激素受体(PR)表达、表皮生长因子受体-2(HER2)表达无相关性(P>0.05),PTN高表达组TNM分期更高,分化程度更低(P<0.05)。结论:血清PTN可以作为乳腺肿瘤的辅助诊断,组织PTN表达能够为乳腺肿瘤预后评估提供帮助。
Objective：To explore the expression level of pleiotrophin（PTN）in sera and tumor tissues of breast cancer and its clinical significance for diagnosis and prognosis.Method：A total of 120 cases of breast cancer patients（observation group）and 100 cases of healthy people（control group）were selected as research object,the elbow vein blood（3 ml）was collected in each person,the content of serum PTN was detected by enzyme linked immunosorbent assay,serum CA15-3 content was detected by chemiluminescence immunoassay,the content of serum CEA was detected by electrochemiluminescence assay,the diagnosis value of serum PTN,CA15-3,CEA were performed by ROC curves.The tumor tissues and adjacent tissues were collected during operation in observation group,and the expression level of PTN in tumor tissue was detected by immunohistochemical staining technique.According to the expression of PTN,breast cancer patients were divided into high expression group and low expression group,and the relationship between PTN expression and prognosis of patients were analyzed.Result：The serum levels of PTN,CA15-3 and CEA in the observation group were significantly higher than those in control group,the difference between two groups was statistically significant（P〈0.05）.ROC curve analysis showed that at the optimal critical value of PTN as an indicator for breast cancer diagnosis,the area under the curve（AUC）was 0.947（95%CI：0.916-0.978,P〈0.05）,the AUC of PTN was higher than CA15-3 and CEA.The expression of PTN in tumor tissue was（7.15±0.49）,and in adjacent tissues was（2.04±0.12）,the expression of PTN in tumor tissue was significantly higher than that in adjacent tissues（P〈0.05）.The postoperative survival time in low expression group was significantly higher than that of high expression group,the difference between two groups was statistically significant（χ^2=4.003,P=0.038）.The expression of PTN was not associated with tumor diameter,lymph node metastasis,ER expression,PR expression and HE
出处
《临床血液学杂志（输血与检验）》
CAS
2017年第6期954-958,共5页
Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)
关键词
多效蛋白
乳腺肿瘤
诊断
预后
pleiotrophin
breast cancer
diagnosis
prognosis
