摘要
大别山独特的地理环境为杜鹃花资源提供了独特的生境地,是中国杜鹃花资源重要的多样性分布中心。该研究以大别山黄杜鹃、云锦杜鹃、满山红3个种群为试验材料,采用9个SSR标记对其进行扩增,以期探究种群遗传多样性和遗传结构的特征。结果表明:9个标记位点共扩增出45个等位基因,每个位点的等位基因数为3~7;3个种群中Shannon信息指数平均值为0.87~0.91;平均观察杂合度和期望杂合度为0.523~0.651和0.517~0.536;总近交系数Fit和种群内近交系数Fis平均值分别为0.075 9和-0.173 3,即3个种群内均存在近交现象;9个位点的平均基因流为0.927 0;在聚类分析中,黄杜鹃和云锦杜鹃亲缘关系最近,与满山红最远。该研究为杜鹃花属植物的进化研究奠定了基础。
Dabie Mountains provides unique habitat for Rhododendron resources,and is an important diversity distribution center of Rhododendron genus in China.In this study,three Rhododendron species,including Rhododendron molle（Blume） G.Don,Rhododendron fortunei Lindl.,and Rhododendron mariesii Hemsl.et Wils.,were selected for genetic diversity and genetic structure analysis with 9 microsatellite markers.The results showed that 45 alleles were obtained,and observed number of alleles per locus ranged from 3 to 7.Shannon index ranged from 0.87 to 0.91.Moreover,HO and HE were in the ranges of 0.523-0.651 and 0.517-0.536,respectively.The mean value of Fit and Fis were 0.075 9 and-0.173 3,respectively,indicating inbreeding took place in all three populations.The average gene flow at each locus was 0.927 0.In the cluster analysis,R.molle was clustered with R.fortunei,inferring closer relations between these two species.This study would lay the foundation for the evolutionary study of Rhododendronspecies.
作者
王书珍
艾莎
张霖
李志良
向福
金卫斌
WANG Shuzhen;AI Sha;ZHANG Lin;LI Zhiliang;XIANG Fu;JIN Weibin(Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, College of Life Science, Huanggang Normal University, Huanggang, Hubei 43800)
出处
《北方园艺》
CAS
北大核心
2017年第24期99-103,共5页
Northern Horticulture
基金
国家自然科学基金资助项目(NSFC31500995)
大别山特色资源开发湖北省协同创新中心团队资助项目(2015TD07)
经济林种质改良与资源综合利用湖北省重点实验室开放基金资助项目(2017BX06)
关键词
杜鹃花
遗传多样性
遗传结构
微卫星
亲缘关系
Rhododendron
genetic diversity
population structure
microsatellite
relationship
