摘要
目的:通过siRNA干扰技术沉默络蛋白激酶2-作用蛋白1(CKIP-1)基因,以观察其对大鼠牙周炎发生发展的影响。方法:取SPF级SD大鼠9只,并将其随机分为空白对照组(BC组)、阴性对照组(NC组)、阳性对照组(PC组)(n=3)。其中NC、PC两组均采用双侧上颌第二磨牙细线结扎和注射LPS-PG的方法建立牙周炎模型,并在造模期间对PC组注射CKIP-1 siRNA干扰;NC组注射生理盐水作为对照。造模加干预4周后,分别对所有大鼠的双侧上颌骨进行Micro-CT扫描,分析其骨缺损高度和根分叉处牙槽骨体积的变化;然后再取其双侧上颌骨标本并制作近远中向牙周牙体组织联合切片,常规HE染色观察其牙槽骨破坏及牙周组织炎症反应情况。结果:Micro-CT扫描显示:NC组的骨缺损程度大于PC组,其牙槽骨吸收高度(mm)分别为0.9606±0.1112、0.6759±0.0514,根分叉处牙槽骨体积(%)分别为0.5958±0.014、0.7509±0.0355(P<0.05)。组织学观察显示:相比于PC组,NC组大鼠的牙槽骨吸收更明显,且牙周膜纤维排序紊乱。结论:通过siRNA干扰技术沉默CKIP-1基因后,可减少大鼠牙周炎模型的骨缺损破坏程度及炎症反应。
AIM： To study the silence of Casein kinase 2-interacting protein 1（ CKIP-1） gene by siRNA interference on the development of periodontitis in rats. METHODS： 9 SD rats were randomly divided into 3 groups（ n = 3）. The rats in group NC and group PC were treated by ligation and injection of LPS-PG at second maxillary molars for the establishement of periodontitis modelse. During the construction of the model,the rats in PC group were injected with CKIP-1 siRNA,and in NC group with saline as a control. The rats without treatment were used as the blank controls（ BC goup）. 20 and 30 d after set-up of the experement Micro-CT and HE staining was performed to campare the damage of alveolar bone and the inflammatory response of the rats among the groups. RESULTS： The decrement of alveolar bone height in NC group was much more than that in PC group, alveolar bone absorption（ mm） ：0. 9606 ± 0. 1112 vs 0. 6759 ± 0. 0514; alveolar bone volume percentage（ %） ： 0. 5958 ± 0. 014 vs 0. 7509 ± 0. 0355（ P 0. 05）. The alveolar bone absorption and the periodontal fiber sorting disorder was more obvious in the NC group than in the PC group. CONCLUSION： Silencing of CKIP-1 gene by siRNA interference can reduce the bone loss and inflammation in the rats with periodontitis.
出处
《牙体牙髓牙周病学杂志》
CAS
2017年第10期563-568,共6页
Chinese Journal of Conservative Dentistry
基金
国家自然科学基金(81470742)
