摘要
Western Blot分析技术是定性和定量检测蛋白的常用技术,其操作流程已很成熟;但我国很多分子生物学实验室起步较晚,其操作还存在较大困难。针对Western Blot实验效果不理想的情况,该文以动物细胞样品为研究对象,通过反复地摸索和分析,建立了一套细胞样品进行Western Blot分析的技术操作流程。实践表明,本研究结果为本实验室的Western Blot分析解决了操作技术问题,同时也为本实验室进行Western Blot分析建立了技术规范。
Western Blot （WB） is a common technique detection of protein commonly used technology which is used for detecting the qualitative and the quantitative of proteins, and the operation process has been skilled. Although the protocol for WB has been widely used, but there are still a lot of operation difficulties in many molecular biology laboratories in China which were relatively late in the studies on molecular biology. Aiming at effect of Western Blot experiment,this paper took the animal cell sample as the research object, through the repeated exploration and analysis, and established the technical operation of Western Blot analysis. Practice shows that the results of this study for the laboratory of Western Blot analysis can solve the operational problems,and also can establish the technical specifications.
作者
汤加勇
赵华
TANG Jiayong;ZHAO Hua(Key Lab oratory of Sichuan Province of Animal Nutrition Institute, Sichuan Agricultural Lniversity, Ya5an 625014, China)
出处
《实验科学与技术》
2017年第6期53-55,75,共4页
Experiment Science and Technology
基金
四川农业大学双支计划(03572073)
