摘要
骨软骨缺损的治疗一直是骨科医生面临的棘手问题,组织工程学的发展为其治疗带来了新的希望.本研究探讨了组织工程材料治疗骨软骨缺损的研究进展.在中国知网、PubM ed和万方数据库中以"骨软骨缺损,组织工程支架,osteochondral defects,cartilage repair,scaffold,3D-printing"为检索词检索关于组织工程材料治疗骨软骨缺损的相关文献.最终选择54篇文献进行综述,得出以下结论:(1)种子细胞包括骨髓间充质干细胞、肌肉干细胞、干细胞衍生的外泌体(SC-Exos)、脂肪干细胞、胚胎干细胞等.SC-Exos显示出取代干细胞治疗关节软骨缺损的巨大潜力.(2)骨形态发生蛋白(BMP)、转化生长因子(TGF)、血管内皮生长因子(VEGF)、胰岛素样生长因子等生长因子在骨软骨缺损的修复过程中发挥了重要作用.(3)单相和双相支架在骨软骨缺损修复中存在缺陷,而三相支架比拟天然骨软骨结构更符合组织工程骨软骨复合支架的要求.(4)3D打印技术在制备多相层支架相比于传统方法如发泡法、粒子滤取法、冷冻干燥法、模板法等具有一定优势.
The treatment of osteochondral defects is a major challenge in orthopedic surgery, while the development of tissue engineering techniques bring new hope for it. This study investigated the application of tissue-engineering scaffolds material in the treatment of osteochondral defects. The related articles addressing tissue-engineered techniques in the treatment of osteochondral defects were retrieved by searching in PubMed, CNKI and Wanfang database with such key words ＂osteochondral defects, scaffolds, 3D-printing＂. Finally, 54 eligible literatures were included. The conclusions are as follows： ①The seed ceils include bone mesenchymal stem cells, muscle stem cells, SC-Exos, adipose-derived stem cells, embryonic stem cells and so on. SC-Exos shows the potential for the replacement of stem ceils in the treatment of articular cartilage defects. ②Growth factors such as BMP, TGF, VEGF and IGF play an important role in the repair of osteochondral defects.③The single layer scaffold and bi-layer scaffold have some shortcomings in the repair of osteochondral defects, while compared with the nature bone cartilage structure, tri-layer scaffold is more consistent with the requirements of tissue engineering osteochondral composite scaffold. ④Compared to traditional methods such as foaming, particle filtration, freeze-drying, template method, 3D printing technology has a certain advantage in the preparation of multi-phase scaffolds.
作者
胡宏悻
步子恒
刘忠堂
HU Hong-Xing;BU Zi-Heng;LIU Zhong-Tang(Department of Orthopedics, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China;Department of Joint Surgery, Changhai Hospital Affiliated to Second Mihtary Medical University, Shanghai 200433, China)
出处
《转化医学电子杂志》
2017年第12期11-16,共6页
E-Journal of Translational Medicine
基金
国家自然科学基金面上项目(51673212)
