摘要
目的:筛选在肾透明细胞癌(clear cell renal cell carcinoma,ccRCC)中差异表达的蛋白,并探讨其在肾癌中的临床意义。方法:采用荧光差异双向凝胶电泳联合质谱技术筛选15例ccRCC及癌旁组织中差异表达的蛋白;蛋白质印迹法在45例ccRCC及癌旁组织中检测筛选获得的差异蛋白的表达水平;免疫组织化学法检测152例ccRCC和40例癌旁组织中差异蛋白的表达情况。分析差异表达蛋白与ccRCC患者临床病理特征及预后的关系。结果:荧光差异双向凝胶电泳联合质谱技术筛选结果显示,ccRCC与其癌旁组织中过氧化物酶4(peroxiredoxin 4,PRDX4)蛋白的表达水平差异明显,与癌旁组织相比,PRDX4在肾癌组织中的表达水平明显下调(P<0.05),癌旁组织中PRDX4蛋白的表达水平是其在癌组织中的3.979倍。蛋白质印迹法检测结果提示,PRDX4在ccRCC中的表达水平较其在癌旁组织中的表达水平明显降低(t=12.810,P<0.001)。免疫组织化学法检测结果显示,PRDX4在ccRCC组织中阳性表达率为36.8%(56/152),较癌旁组织的87.5%(35/40)明显降低(χ2=32.594,P<0.001);PRDX4与ccRCC病理分级(χ2=6.802,P=0.033)和远处转移(χ2=3.925,P=0.048)有关,且与病理分级(γ=-0.211,P=0.009)及远处转移(γ=-0.161,P=0.048)呈负相关。PRDX4的表达与性别、年龄、T分期、淋巴结转移及复发无关(P值均>0.05)。PRDX4表达阳性组和表达阴性组5年生存率分别为75.3%和62.7%,差异无统计学意义(P=0.862)。结论:PRDX4作为ccRCC差异表达蛋白可能参与了肾癌的发生和发展,其表达下调与ccRCC恶性程度增高相关。
Objective： To screen the differential renal cell carcinoma （ccRCC）, and to ly expressed proteins in clear cell investigate its clinical significance. Methods： The differentially expressed proteins in 15 cases of ccRCC and their adjacent renal tissues were screened by two-dimensional fluorescence difference in gel electrophoresis combined with mass spectrometry （2-D DIGE-MS）. The expression levels of differentially expressed proteins screened by 2-D DIGE-MS in 45 cases of ccRCC and their adjacent tissues were examined by Western blotting. The expression levels of this differentially expressed proteins in 152 specimens of ccRCC and 40 specimens of adjacent tissues were detected by immunohistochemistry. The correlations of this protein expression with the clinicopathologic features and prognosis of ccRCC patients were analyzed. Results： Proteomics screening results showed that the expression level of peroxiredoxin 4 （PRDX4） was significantly different in ccRCC and its adjacent tissues; compared with the adjacent tissues, PRDX4 expression was significantly down-regulated in ccRCC tissues （P 〈 0.05）; and the expression level of PRDX4 in the adjacent tissues was 3.979 times higher than that in ccRCC tisseus. Western blotting showed that the expression level of PRDX4 in ccRCC tissues was significantly lower than that in adjacent tissues （t = 12.810, P 〈 0.001）. Immunohistochemistry showed that the positive expression rate of PRDX4 in ccRCC tissues was significantly lower than that in the adjacent tissues [36.8% （56/152） vs 87.5% （35/40）, X^2= 32.594, P 〈 0.001]. Furthermore, the expression level of PRDX4 was correlated with tumor grade （X^2= 6.802, P = 0.033） and distant metastasis （X^2 = 3.925, P = 0.048）, but was not related to gender, age, T stage, lymph node metastasis and recurrence （all P 〉 0.05）. The patients with lower expression of PRDX4 had high grade of ccRCC （γ=-0.211, P = 0.009） and distant metastasis （y = -0.161, P = 0.048）. The 5-year survival
作者
陈壮飞
肖耀军
黄泽海
陈彤
秦自科
郑少斌
CHEN Zhuangfei1;XlAO Yaojun2;HUANG Zehai1;CHEN Tong1;QIN Zike3;ZHENG Shaobin1
出处
《肿瘤》
CAS
CSCD
北大核心
2017年第12期1304-1312,共9页
Tumor
