摘要
目的评价白藜芦醇对肠缺血再灌注大鼠急性肺损伤时NADPH氧化酶活性的影响。
方法清洁级健康雌性SD大鼠32只，体重180～230 g，采用随机数字表法分为4组（n＝8）：假手术组（Sham组）、肠缺血再灌注组（I/R组）、溶媒组（Veh组）和白藜芦醇组（Res组）。采用夹闭肠系膜上动脉75 min，恢复灌注4 h的方法制备肠缺血再灌注大鼠肺损伤模型。Res组于造模前连续5 d及缺血前15 min腹腔注射白藜芦醇15 mg/kg。Veh组腹腔注射等容量溶媒（0.5%酒精）。Sham组与I/R组腹腔注射等容量生理盐水。于再灌注4 h时处死大鼠取肺组织，光镜下观察肺组织病理学结果并行损伤评分，确定肺组织湿/干重（W/D）比值，比色法检测MDA含量和SOD活性，Western blot法检测NADPH氧化酶亚型（gp91phox和p47phox）的表达。
结果与Sham组比较，I/R组和Veh组肺组织病理学损伤评分、W/D比值和MDA含量升高，gp91phox和p47phox表达上调，SOD活性降低（P〈0.05）；与I/R组和Veh组比较，Res组肺组织病理学损伤评分、W/D比值和MDA含量降低，gp91phox和p47phox表达下调，SOD活性升高（P〈0.05）。
结论白藜芦醇减轻肠缺血再灌注大鼠肺损伤的机制与其抑制NADPH氧化酶介导的氧化应激反应有关。
Objective To evaluate the effect of resveratrol on the activity of NADPH oxidase during acute lung injury induced by intestinal isehemia-rcperfusion （I/R） in rats, Methods Thirty-two pathogen- free healthy female Sprague-Dawley rats, weighing 180-230 g, were divided into 4 groups （n= 8 each） u- sing a random number table： sham operation group （Sham group） , intestinal I/R group （I/R group） , ve- hicle group （Veh group） and resveratrol group （Res group）. Intestinal I/R was produced by occlusion of the superior mesenterie artery for 75 min followed by 4 h of reperfusion. In group Res, resveratrol 15 mg/kg was intraperitoneally injected for 5 consecutive days before establishment of the model and at 15 rain before ischemia. The equal volume of vehicle （0.5% alcohol） was intraperitoneally injected in group Veh. The e- qual volume of normal saline was intraperitoneally injected in Sham and I/R groups. At the end of reperfu- sion, the rats were sacrificed and the long was removed for microscopic examination of pathologic changes which were scored and for determination of wet/dry weight ratio （ W/D ratio） , malondialdehyde （MDA）content and superoxide dismutase （SOD） activity （ by colorimetric method） and expression of NADPH oxi- dase subunits （gp91phx and p47vhx） in lung tissues （by Western blot）. Results Compared with group Sham, pathologic scores, W/D ratio and MDA content were significantly increased, the expression of gp91hx and p47x was up-regulated, and the activity of SOD was decreased in I/R and Veh groups （P〈 0.05）. Compared with I/R and Veh groups, pathologic scores, W/D ratio and MDA content were signifi- cantly decreased, the expression of gp91phx and p47phx was down-regulated, and the activity of SOD was increased in group Res （P〈0.05）. Conclusion The mechanism by which resveratrol reduces intestinal I/ R-induced acute lung injury is related to inhibiting NADPH oxidase-mediated oxidative stress response of Fats.
出处
《中华麻醉学杂志》
CSCD
北大核心
2017年第9期1139-1141,共3页
Chinese Journal of Anesthesiology
基金
2014年广东省科技计划项目（2014A020212003）
关键词
酚类
再灌注损伤
肠
呼吸窘迫综合征
成人
氧化性应激
Phenols
Reperfusion injury
Intestines
Respiratory distress syndrome,adult
Oxidative stress
