摘要
目的 评价姜黄素预处理对大鼠肠缺血再灌注时黄嘌呤氧化酶活性的影响.方法 清洁级雌性SD大鼠30只,体重180 ～ 220 g,采用随机数字表法,将其分为3组（n=10）：假手术组（S组）、肠缺血再灌注组（I/R组）和姜黄素预处理组（Cur组）.采用夹闭肠系膜上动脉75 min后恢复灌注的方法制备大鼠肠缺血再灌注损伤模型.夹闭肠系膜上动脉前5d,Cur组每天胃饲姜黄素200mg/kg（采用生理盐水稀释）,S组和I/R组胃饲等容量生理盐水.于再灌注4h时放血处死大鼠,取肠组织标本,光镜下观察病理学结果并行Chiu评分,采用分光光度法检测黄嘌呤氧化酶活性,硫酸代巴比妥酸法测定丙二醛含量,黄嘌呤氧化酶法检测超氧化物歧化酶活性.结果 与S组比较,I/R组和Cur组大鼠肠组织Chiu评分、黄嘌呤氧化酶活性、丙二醛含量升高,超氧化物歧化酶活性降低（P＜0.05）；与I/R组比较,Cur组大鼠肠组织Chiu评分、黄嘌呤氧化酶活性、丙二醛含量降低,超氧化物歧化酶活性升高（P＜0.05）.结论 姜黄素预处理可减轻大鼠肠缺血再灌注损伤,可能与其抑制肠组织黄嘌呤氧化酶活性,从而降低氧化应激反应有关.
Objective To evaluate the effects of curcumin preconditioning on the activity of xanthine oxidase （XOD） during intestinal ischemia-reperfusion （I/R） in rats.Methods Thirty female Sprague-Dawley rats,aged 180-220 g,were randomly divided into 3 groups （n =10 each） using a random number table：sham operation group （S group）,intestinal I/R group （I/R group）,and curcumin preconditioning group （Cur group）.Intestinal I/R was induced by clamping the superior mesenteric artery for 75 min followed by reperfusion.Curcumin 200 mg/kg was given everyday for 5 days before intestinal I/R in Cur group and the equal volume of normal saline was given instead of curcumin in S and I/R groups.The rats were sacrificed at 4 h of reperfusion and the intestinal specimens were obtained for microscopic examination of pathologic changes which were graded using Chiu scoring system and for determination of XOD activity,content of malondialdehyde （MDA）,and superoxide dismutase （SOD） activity.Results Compared with S group,the Chiu score,activity of XOD and content of MDA were significantly increased,while the activity of SOD was decreased in I/R and Cur groups （P 〈 0.05）.Compared with I/R group,the Chiu score,activity of XOD and content of MDA were significantly decreased,and the activity of SOD was increased in Cur group （P 〈 0.05）.Conclusion Curcumin preconditioning can attenuate intestinal I/R injury in rats,which may be due to inhibition of XOD activity and decreased oxidative stress in intestinal tissues.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2014年第1期102-104,共3页
Chinese Journal of Anesthesiology
基金
广东省卫生厅医学科研基金（A2013673）
关键词
姜黄素
再灌注损伤
肠
黄嘌呤氧化酶
Curcumin
Reperfusion injury
Intestines
Xanthine oxidase
