摘要
近年来,大量证据表明长链非编码RNA(long non-coding RNAs,lncRNAs)在肿瘤发生发展中发挥重要的作用。本研究通过生物信息学预测发现,核仁小分子RNA宿主基因1(small nucleolar RNA host gene 1,SNHG1)无蛋白质编码功能,且主要定位于细胞质。qRT-PCR结果显示,SNHG1在肺癌细胞中的表达量显著高于正常肺上皮细胞。在肺癌细胞NCI-H1299中,敲低SNHG1发现,该细胞增殖能力明显下降;在正常肺上皮细胞BEAS-2B中,过表达SNHG1可显著促进该细胞的增殖能力。深入研究发现,SNHG1可上调CDK4和下调p27kip1在蛋白质水平的表达,而对其mRNA水平表达量无影响。此外,在肺癌临床组织中也发现SNHG1高表达,且高表达的SNHG1与肺癌瘤体大小、TNM分期和远端转移正相关,而与患者年龄及吸烟史无关。综上所述,SNHG1在肺癌中高表达,通过上调CDK4和下调p27kip1推进肺癌进程。
Recently,accumulating evidences suggest that long non-coding RNAs（lncRNAs） play important roles in cancer development and progression.In the present study,data from bioinformatics analysis found that small nucleolar RNA host gene 1（SNHG1） was located in cytoplasm with no protein coding ability.Results from qRT-PCR assay demonstrated that SNHG1 was highly expressed in lung cancer cells when compared with lung epithelial cells.Moreover,Brd U assay suggested that the proliferation of BEAS-2B lung epithelial cells was significantly enhanced by overexpression of SNHG1.Similarly,knock down of SNHG1 in NCI-H1299 lung cancer cells could extremely suppress cells proliferation.SNHG1 could obviously accelerate CDK4 and inhibit p27kip1 expression at protein level but had no effect at mRNA level.In addition,results showed that SNHG1 expression was increased in 43 cases of lung cancer tissues when compared with adjacent normal tissues.SNHG1 expression was positively correlated with tumor size,TNM stage and metastasis of lung cancer.In conclusion,SNHG1 is highly expressed in lung cancer and promotes lung cancer progression through elevating CDK4 and reducing p27kip1 expression at protein level.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
北大核心
2017年第12期1274-1279,共6页
Chinese Journal of Biochemistry and Molecular Biology
基金
广东省清远市科技项目(No.2012B011204028)资助~~
关键词
长链非编码RNA
核仁小分子RNA宿主基因1
肺癌
增殖
long non-coding RNA （lncRNA）
small nucleolar RNA host gene 1 （SNHG1）
lung cancer
proliferation
