摘要
目的探讨核桃肽(WP)对幼年小鼠学习记忆能力的改善作用及其机制。方法选用北京大学医学部实验动物科学部提供的48只初断乳C57BL/6J雄性小鼠,随机分成4组,空白对照组和低(110 mg/kg·bw)、中(220 mg/kg·bw)、高(440 mg/kg·bw)3个剂量WP组,每组12只,干预30 d后进行开阔场实验、Morris水迷宫实验、跳台实验和穿梭箱实验,观察小鼠的自主活动能力及情绪反应、空间记忆能力和主动及被动回避能力。结果Morris水迷宫结果显示,WP高剂量组小鼠的逃避潜伏期较空白对照组明显缩短,WP中、高剂量组在目标象限停留时间和穿台次数较空白对照组明显增加,差异均有统计学意义。跳台实验24 h测试中,与空白对照组相比,WP低、高剂量组错误次数减少,三个干预组停留潜伏期均增长,5 d后测试中,WP中剂量组对电击的记忆消退较慢,差异均有统计学意义。穿梭箱实验中,与空白对照组相比,WP低、中、高干预组小鼠的主动回避次数增多,受到电击次数减少。WP剂量组血清T-SOD及GSH-Px活性高于空白对照组,MDA水平低于空白对照组,差异均有统计学意义。结论 WP可以增强幼年小鼠学习和空间记忆能力、主动及被动回避能力,也可能具有延缓小鼠学习记忆的衰退的作用,其机制可能与WP的抗氧化性有关。
Objective To investigate the improvement effects and mechanisms of walnut peptides （WP） on learning and memory abilities in juvenile mice. Methods 48 male early weaning C57BL/6J mice （provided by Department of Laboratory Animal Science, Peking University Health Science Center） were randomly divided into 4 groups： the control group, low （110 mg/kg ·bw）, middle （220 mg/kg · bw）, and high WP intervention group（440 mg/kg· bw）, with 12 mice in each group. After 30 days feeding, the Open-field test, Morris Water Maze, Step-down test and Shuttle test were performed in those mice, to observe the ability of autonomic activities and emotional responses, spatial memory ability, and the active and passive avoidance reactions. Results The results of Morris Water maze showed that the escape latency of high WP group was significantly reduced, and the duration staying in the target quadrant and the frequency of crossing over the platform among middle and high WP groups was significantly increased compared with the control group. The Step-down test indicated three intervention groups had longer step-down latency, less errors in the low and high WP groups than the control group. After 5 days of training, the memory of shocking in the middle WP group declined statistically slower than control group. Shuttle test revealed that the numbers of active avoidance reaction increased, while the times of electric shock decreased among three intervention groups compared with the control group. The serum T-SOD and GSH-Px concentrations in three WP groups were higher and the level of MDA was lower than the control group, and the difference were all statistically significant. Conclusion WP could enhance the abilities of learning and spatial memory, active and passive avoidance, and may also retard the decline of learning and memory in juvenile mice. The mechanism may be related to the antioxidant activity of WP.
出处
《中国生育健康杂志》
2017年第6期538-543,共6页
Chinese Journal of Reproductive Health
基金
中国博士后科学基金(2016M591026)
关键词
核桃肽
幼年小鼠
学习记忆能力
Walnut Peptides
Juvenile mice
Learning and memory ability
