摘要
目的:观察针刺联合中药对未成年雌性大鼠的动情周期、血清卵泡刺激素(FSH)、黄体生成素(LH)及孕激素(P)的影响。方法:未成年健康雌性SD大鼠40只,随机分成4组:即空白对照组、针刺组、中药组、针药结合组,每组10只。空白对照组:生理盐水灌胃。针刺组:取穴气海、关元、中极、肾俞、次髎、三阴交、太冲穴,进针0.3 cm,行平补平泻法,留针20 min。中药组:补肾健脾方水煎剂灌胃。针药组:补肾健脾方水煎剂灌胃合用针刺。观察经处理后各组大鼠动情周期出现情况以及血清FSH、LH及P水平的变化。结果:与空白对照组比较,针刺组、中药组和针药结合组大鼠出现动情周期例数显著升高,血清FSH、LH、P的水平显著升高,差异均有统计学意义(P<0.05),提示针刺、中药能改善性腺生殖轴。与针药结合组比较,针刺组和中药组大鼠血清FSH、LH、P的水平较低,差异均有统计学意义(P<0.05)。结论:针药合用能明显提高未成年大鼠血清FSH、LH及P水平,表明针药合用可以促进卵泡发育及排卵,从而可以使增殖期内膜转化为分泌期,因而可以用于青春期功血的治疗。
Objective： To observe the effect of acupuncture combined with Chinese medicine for estrous cycle, serum follicle stimulating hormone（FSH）, luteinizing hormone（LH）, and pregesterone（P） of impuberal female rats. Methods： Divided 40 healthy impuberal female SD rats into four groups, namely blank control group, acupuncture group, Chinese medicine group, and the combination group, 10 rats in each group. Blank control group was given normal saline by gavage. The acupuncture group received uniform reinforcing-reducing by 0.3 cm inserting needles at Qihai, Guanyuan, Zhongji, Shenshu, Ciliao, Sanyinjiao, Taichong for 20 min once a day. Chinese medicine group was given Bushen Jianpi prescription by gavage. The combination group was given both therapies of acupuncture group and Chinese medicine group. Observed estrous cycle as well as changes of levels of FSH, LH and P after treatment. Results： Compared to blank control group, numbers of estrous cycle among the other three groups were obviously improved, as well as levels of serum FSH, LH and P, differences being significant （P 〈0.05）, which indicated that acupuncture and Chinese medicine could improve the sex gland axis. Levels of serum FSH, LH and P in acupuncture group and Chinese medicine group were lower than those in the combination group, differences being significant（P 〈0.05）. Conclusion： The therapy of acupuncture combined with Chinese medicine can evidently improve levels of serum FSH, LH and P of impuberal rats, which suggested that the therapy can stimulate follicular development and ovulation to change the inner membrane from multiplication period to secretory period. Therefore, the combination therapy can be applied in the treatment of adolescent dysfunctional uterine bleeding.
出处
《新中医》
CAS
2017年第12期11-13,共3页
New Chinese Medicine
基金
广东省中医药局项目(20141211)
关键词
针刺
中药
青春期功血
动情周期
性腺轴
动物实验
大鼠
Acupuncture
Chinese medicine
Adolescent dysfunctional uterine bleeding
Estrous cycle
Sex gland axis
Animal experiment
Rats
