摘要
目的利用3种方法建立糖尿病大鼠血糖波动模型并进行比较.方法 75只SD大鼠随机分成正常组(n=15)和模型组(n=60),高糖高脂饲料喂养模型组SD大鼠6周后用小剂量链脲佐菌素(STZ)30 mg/kg腹腔注射诱导建立糖尿病模型,造模成功后将其随机分成持续高糖组(n=15)、波动A组(n=15)、波动B组(n=15)和波动C组(n=15).波动A组大鼠每日2次腹腔注射50%葡萄糖,波动B组每日2次皮下注射普通胰岛素,波动C组每日2次腹腔注射50%葡萄糖+每日2次皮下注射普通胰岛素,建立糖尿病大鼠血糖波动模型.观察大鼠"三多一少"症状,血糖变化情况.结果 (1)血糖波动组和持续高糖组的"三多一少"症状明显;(2)实验结束时,持续高糖组存活13只,存活率86.7%,波动A组存活10只,存活率66.7%,波动B组存活11只,存活率73.3%,波动C组存活8只,存活率53.3%.血糖波动C组与正常组和持续高糖组相比,存活率明显下降(P分别为0.003,0.046),但与波动A组和波动B组相比差异无统计学意义(P>0.05);(3)血糖波动A组、血糖波动B组、血糖波动C组的SDBG、LAGE均高于持续高糖组[SDBG:波动A组(3.51±0.59)mmol/L、波动B组(3.97±0.59)mmol/L、波动C组(6.43±0.45)mmol/L vs(1.84±0.55)mmol/L;LAGE:A组(8.92±1.22)mmol/L、波动B组(10.45±1.88)mmol/L、波动C组(16.92±1.16)mmol/L vs(5.10±1.50)mmol/L];(4)与其他两组血糖波动组相比,血糖波动C组SDBG、LAGE明显升高[SDBG:(6.43±0.45)mmol/L vs(3.51±0.59)mmol/L vs(3.97±0.59)mmol/L;LAGE:(16.92±1.16)mmol/L vs(8.92±1.22)mmol/L vs(10.45±1.88)mmol/L].结论 3种方法均能成功建立糖尿病大鼠血糖波动模型,腹腔注射葡萄糖结合皮下注射胰岛素的方法造成的血糖波动更大,且存活率无明显降低.
Objective To compare three modeling methods in Blood Glucose Fluctuation of Diabetic Rats.Methods The 75 male Sprague-Dawley（SD ） rats were randomly assigned into two groups,normal group （n=15） and model group （n=60） .Normal group rats were fed with normal diet,model group rats were fed withahigh-sucrose-high-fat diet.After 6 weeks,a low dose of streptozotocin （STZ,30 mg/kg ） was injected to inducehyperglycemia in model group rats.And then the model group rats were randomly divided into four subgroups：diabetic group （n=15 ）, groupA （n=15）, groupB （n=15） and groupC （n=15） . Group A rats wereinduced by intraperitoneal injecting 50% glucose by 0.38g/kg twice daily at 8 o＇clock and 14 o＇clock.GroupB ratswere induced by subcutaneous injecting insulin at 8：30 and 14：30. GroupC were induced by intraperitoneal injecting 50% glucose by 0.38g/kg at 8 o＇clock and 14 o＇clock and subcutaneous injecting insulin at 8：30 and 14：30. Diabeticgroup were induced by intraperitoneal injecting 0.9% saline.All rats were executed after intervening for 6 weeks. Foodintake, weight, urine and blood glucose were observed during the experiment. Results （1） More drinking,more eating,more urine and low weight were observed in diabetic group,groupA,group B and group C. （2） Atthe end of the experiment,15 rats were alive in the normal group, the survival rate was 100%.13 rats were alive inthe diabetic group,the survival rate was 86.7%. 10 rats were alive in groupA, the survival rate was 66.7%. 11 ratswere alive in groupB,the survival rate was 73.3%.8 rats were alive in groupC, the survival rate was 53.3%. Thedifference between the five groups was statistically significant （ P= 0.03） . The survival rate of groupC wassignificantly lower than that of normal group and diabetic group （ P= 0.003,P = 0.046, respectively ）, but therewas no significant difference compared with group A and groupB （ P〉0.05） . （3） Compared with the diabeticgroup,SDBG and LAGE were significantly increa
出处
《昆明医科大学学报》
CAS
2017年第12期12-17,共6页
Journal of Kunming Medical University
基金
江苏省自然科学基金资助项目(BK20131092)
关键词
糖尿病
血糖波动
模型
大鼠
Diabetes mellitus
Blood glucose fluctuation
Model
Rats
